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Development of Bio-imaging Mouse Model for Efficacy of New Drug Candidates

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.4-4
  • 저자
    Hee-Young Yang, Sung-Gon Kim, Byeong-Jin Park, Sang-Kyoon Kim, Sang-Joon Park, Kil-soo Kim, Gabbine Wee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347413

원문정보

초록

영어
Laboratory animals more than 100 million have consumed annually in laboratories for test of chemicals, medical devices, drugs and foods, and also have brought about a social problem in aspect of bioethics. Recently, bio-imaging system that can detect the fluorescence or bioluminescence signals in vivo, has considered as a replacement solution in the use of laboratory animals. Here, we tried to construct a mouse model with BRET(Bioluminescence Resonance Energy Transfer) system that is programmed to cut a recognizable site (DEVD amino acid sequence) by caspase-3 when the drug efficacy is observed. BRET expression vector was composed of a bioluminescent luciferase and a red fluorescent protein (tdTomato) under CAG promoter, and DEVD sequence was inserted between their proteins. Luciferase and tdTomato expressed normally in BRET-PC3 human prostatic cancer cells, and red fluorescence was observed under radiation- free status. During drug inducible apoptosis, bioluminescence rate catalyzed by luciferase rapidly increased whereas red fluorescent intensity decreased slightly. Next, BRET-PC3 cells injected into underskin in the femoral region to produce xenograft mouse model and were identified successfully expectable events occurred after treatment of apoptotic drugs like as BRET-PC3 cells. Finally, we constructed mouse expressing BRET system by microinjection and confirmed strong detectable signals using bio-imaging equipment. In this study, we developed a new mouse model with BRET system that can detect apoptotic reagents, and suggest the possibility as a teat model for drug discovery and development.

키워드

Bio-imaging BRET Mouse model Drug development Apoptosis

저자

  • Hee-Young Yang [ Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu, Republic of Korea ]
  • Sung-Gon Kim [ Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu, Republic of Korea ]
  • Byeong-Jin Park [ Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu, Republic of Korea ]
  • Sang-Kyoon Kim [ Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu, Republic of Korea ]
  • Sang-Joon Park [ Colleage of Veterinary medicine, Kyungpook National University, Daegu, Republic of Korea ]
  • Kil-soo Kim [ Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu, Republic of Korea, Colleage of Veterinary medicine, Kyungpook National University, Daegu, Republic of Korea ]
  • Gabbine Wee [ Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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