Earticle

현재 위치 Home

Poster Presentation : Transgenesis / Xenotransplatation

Effect of US11 and hDAF Gene for Cytotoxicity Regulate and Transgenic Pigs Breeding

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.159-159
  • 저자
    Hyeon Yeong Shin, Tao Lin, Reza K. Oqani, Jae Eun Lee, Jung won Kang, Joo Bin Lee, Dong Il Jin
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347410

원문정보

초록

영어
This study aims to find effect of natural mating and cytotoxicity regulate from US11 and hDAF gene transgenic pig. Piglets that has US11, hDAF or US11&hDAF genes were born from natural mating of female US11 gene transgenic pig and male hDAF gene transgenic pig. Among 10 piglets were born, there are 2 piglets with US11 gene only, 2 piglets with hDAF gene only, 5 piglets with both US11 and hDAF gene, and 1piglets without transgenes. Sex ratio of piglets was 1:1, and transfection rate from natural breeding showed result that only US11 gene was 20%, only hDAF gene was 20%, and both US11 gene and hDAF gene was 50%. In cytotoxicity effect, US11 gene was showed a tendency of reduce human NK cell cytotoxicity in 10:1 ratio. In complement assay, hDAF gene was reduce human complement cytotoxicity in both 1:1 and 10:1 ratio of human complement. hDAF gene was also effected in reduce human NK cell cytotoxicity that showed similar result with US11 gene. Double transgenic piglets fibroblast that has both US11 and hDAF resulted that decrease both human NK cell and complement cytotoxicity compared with wild type cells. In conclusion, Natural mating was effected for US11 and hDAF gene transmitted into offsping piglets, and US11 and hDAF gene were expected to regulate xenograft rejections in transgenic tissues.

키워드

US11 hDAF NK cell Immune toxicity

저자

  • Hyeon Yeong Shin [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
  • Tao Lin [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
  • Reza K. Oqani [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
  • Jae Eun Lee [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
  • Jung won Kang [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
  • Joo Bin Lee [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]
  • Dong Il Jin [ Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 The 17th International Symposium on Developmental Biotechnology

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.