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Poster Presentation : Transgenesis / Xenotransplatation

Production of Human FGF2 Knock-in Bovine Embryos using Microinjection

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.156-156
  • 저자
    Min-Jee Park, Eun-Young Kim, Se-Pill Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347408

원문정보

초록

영어
The production of transgenic livestock has the opportunity to significantly improve human health, enhance nutrition, protect the environment, increase animal welfare, and decrease livestock disease. In this study, bovine PN embryos were injected in cytoplasm with three groups (Ⅰ: sgRNA+Ca9 protein+KI_hFGF_GFP vector, Ⅱ: sgRNA+Ca9 mRNA+KI_hFGF_GFP vector and Ⅲ: bCNS_EX3_sgRNA+pBSK_sgRNA+Ca9 protein+ KI_hFGF_GFP vector) at 18~20 h after IVF, and treated 7.5 uM RS-1 (RAD51-stimulatory compound 1) for 24 h after microinjection. The survival rate post day 2 was higher in group Ⅰ (84.4%) than other groups. At day 4, embryo development potential (78.1%) and GFP expression was significantly highest in group Ⅲ (54.5%) among treatment groups (p<0.05). At day 8, while the blastocyst formation was significantly high in group Ⅰ(32.1%) among three groups, the GFP expression embryo rate of developed blastocyst was higher in group Ⅱ (34.8%) and Ⅲ (38.5%) than in group Ⅰ (17.1%). The total injection efficiency of GFP embryo production from gene injected embryos was a little higher in group I (5.5%, 6/109) than in group II (4.1%, 8/194) and group III (3.6%, 5/139). However, the GFP expressed healthy embryos were the more presented in group II than other groups. Also, when the effect of 7.5 uM RS-1 treatment to the injected embryos for 24 h were examined, the survival rate post day 2 was higher in RS-1 treatment group (72.5%) than in control group (67.3%). The GFP expression rate was also higher in RS-1 treatment group (57.6% and 58.3%) than in control group (38.8% and 19.2%) at day 4 and 8 development in vitro. This study demonstrates that it can be helpful to the production of hFGF knock-in bovine embryos using combination of bCNS_EX3_sgRNA+pBSK_sgRNA+Ca9 protein+KI_hFGF_ GFP vector and treatment with RS-1.

키워드

Bovine hFGF Knock-in Microinjection RS-1

저자

  • Min-Jee Park [ Jeju National University, Stem Cell Research Center, Jeju National University, Mirae Cell Bio ]
  • Eun-Young Kim [ Jeju National University, Stem Cell Research Center, Jeju National University, Mirae Cell Bio ]
  • Se-Pill Park [ Jeju National University, Stem Cell Research Center, Jeju National University, Mirae Cell Bio ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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