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Poster Presentation : Transgenesis / Xenotransplatation

Characterization of Transgenic Cells Knocked-out with Hyperacute Immune Rejection Genes

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.151-151
  • 저자
    Seung-Chan Lee, Ju Yeon Lee, In-Sul Hwang, Mi-Ryung Park, Keon Bong Oh, Sun-A Ock, Jae-Seok Woo, Gi-Sun Im, Seongsoo Hwang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347403

원문정보

초록

영어
The α-Gal epitope (Galα1,3Galα1,4GlcNAc-R), created by alpha-1,3-glycosyltransferase-1 (GT), is recognized by natural anti-α-1,3-galactose antibodies in human serum and responsible for hyperacute rejection (HAR) in pig-to-human/primate xenotransplantation. Although, the generation of GT knockout (KO) pigs has overcome hyperacute rejection, an isoglobotriosylceramide synthase (iGb3s) may produce additional alpha-1,3-terminated galactose residues on glycosphingolipid. This study was performed to investigate effects of iGb3s KO on the glycoproteomic characterization in the transgenic pig cells. We established and used 2 different transgenic cells such as GT-MCP/-MCP (GT/MCP) and GT-MCP/-MCP+iGb3s—/— (GT/MCP/iGb3s) cells. WT pig cells were used as a control. To characterize the expression of α-Gal epitope, we analyzed the cells by FACS using BS-I-B4 lectin antibody. The α-Gal epitope expression in all both TG cells was significantly down regulated as compared with WT (p<0.05). The iGb3s protein level was decreased in GT/MCP/iGb3s cells compared with GT/MCP cells. To analyze changes of glycoproteome level, LC-MS/MS analysis was performed in WT, GT/MCP, and GT/MCP/iGb3s cells. A total amount of glycopeptide in GT/MCP/iGb3s was increased as compared with that of GT/MCP. However, glycopeptide forms of gal-gal were reduced in GT/MCP/ iGb3s compared to both WT and GT/MCP. Our results demonstrated that additional KO of iGb3s gene reduced the expressions of α-Gal epitope and glycopeptide forms of gal-gal in GT/MCP. Further studies are needed to evaluate synergic effects of double gene knock out in order to minimize a HAR response after xenotransplantation.

키워드

Xenotransplantaion Hyperacute rejection α-Gal

저자

  • Seung-Chan Lee [ Animal Biotechnology Division, National Institute of Animal Science, Wanju, Korea ]
  • Ju Yeon Lee [ Biomedical Omics Group, Korea Basic Science Institute, Ochang, Korea ]
  • In-Sul Hwang [ Animal Biotechnology Division, National Institute of Animal Science, Wanju, Korea ]
  • Mi-Ryung Park [ Animal Biotechnology Division, National Institute of Animal Science, Wanju, Korea ]
  • Keon Bong Oh [ Animal Biotechnology Division, National Institute of Animal Science, Wanju, Korea ]
  • Sun-A Ock [ Animal Biotechnology Division, National Institute of Animal Science, Wanju, Korea ]
  • Jae-Seok Woo [ Animal Biotechnology Division, National Institute of Animal Science, Wanju, Korea ]
  • Gi-Sun Im [ Animal Biotechnology Division, National Institute of Animal Science, Wanju, Korea ]
  • Seongsoo Hwang [ Animal Biotechnology Division, National Institute of Animal Science, Wanju, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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