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Poster Presentation : Transgenesis / Xenotransplatation

The Expression of Hyperacute Immune Rejection Genes in the Skin derived from Transgenic Pigs

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.149-149
  • 저자
    Seung-Chan Lee, In-Sul Hwang, Tae-Uk Kwak, Mi-Ryung Park, Keon Bong Oh, Sun-A Ock, Jae-Seok Woo, Jeong-Woong Lee, Gi-SunIm, Seongsoo Hwang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347401

원문정보

초록

영어
Porcine skin and preserved cadaver skin are used for temporary wound coverage, but 1 to 2 weeks after grafting, these tissues undergo immune-mediated rejection. To avoid such antigenicity, different types of acellular dermal matrix (ADM) have been studied. The generation of pigs lacking the α-1,3 galactosyl transferase (GT—/—) expressed by α-Gal epitope (Galα1,3Galα 1,4GlcNAc-R) has minimized hyperacute rejection. This study was performed to investigate the expression of α-Gal epitope in the skin derived from GT—/— transgenic pigs. The skin (7/1,000 inches) was obtained by dermatome (Zimmer®Electric Dermatome) from around one month old of wild type (WT), GT—/—,and GT-MCP/-MCP piglets, respectively. The skins treated with decellularization were fixed, dehydrated, cleaned, and embedded. It was confirmed that α-Gal epitope was not detected in the skins from TG pigs stained with BS-IB4 lectin antibody, but in WT skin. To analyze the characteristics of immune response genes in ADM skins, the tissue sections were stained with anti-HLAE and anti-MHC I antibodies. Decellularized skins of GT—/—and GT-MCP/-MCP were not stained in both HLAE and MHC I, but the both immune response genes were strongly expressed in epidermis and dermis, respectively. Taken together, it can be postulated that ADM skin originated from GT KO pigs may be used as a source of xenoskingraft. Further studies are needed to evaluate the functions of the ADM skins of GT—/— and GT-MCP/-MCP pigs after xenograft.

키워드

Xenotransplantaion Hyperacute rejection Skingraft

저자

  • Seung-Chan Lee [ Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Republic of Korea ]
  • In-Sul Hwang [ Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Republic of Korea ]
  • Tae-Uk Kwak [ Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Republic of Korea ]
  • Mi-Ryung Park [ Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Republic of Korea ]
  • Keon Bong Oh [ Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Republic of Korea ]
  • Sun-A Ock [ Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Republic of Korea ]
  • Jae-Seok Woo [ Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Republic of Korea ]
  • Jeong-Woong Lee [ unctional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea ]
  • Gi-SunIm [ Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Republic of Korea ]
  • Seongsoo Hwang [ Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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