Earticle

현재 위치 Home

Poster Presentation : Transgenesis / Xenotransplatation

Construction of Knock-in Vector for the Production of Human Tissue Plasminogen Activator Protein on the Porcine Whey Acid Protein Gene Locus

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.145-145
  • 저자
    Ji-Youn Kim, Mi-Ryung Park, Hak-Jae Chung, SungJune Byun, KeonBong Oh, Hyeon Yang, Dong Hoon Kim, Kyung-Woon Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347397

원문정보

초록

영어
The recombinant production of therapeutic proteins from transgenic animal is the major technology of animal biotechnology and is one of the biggest challenges in an efficient bioreactor in the pharmaceutical industry. The useful gene delivery into cells or animals is an important way to express the target gene in mammary glands. Tissue plasminogen activator (tPA) is known to play an important role in the major enzyme responsible for blood clots breakdown. Because it works on the clotting system, tPA is used in clinical medicine to treat thrombolysis, such as pulmonary embolism, myocardial infraction and stroke. The objective of this study is a production of recombinant human tPA protein in the milk of porcine. For this study, we construct knock-in vector that human tPA gene is inserted into the exon 1 locus of porcine whey acid protein (pWAP) gene knock-out somatic cell by homologous recombination. Finally, establishment of tPA knock-in cell lines were confirmed by genomic PCR analysis and identification of karyotype. As a result, we established cell lines of pWAP knock-out expressing human tPA gene from porcine fibroblasts to apply somatic cell nuclear transfer (SCNT). Further study will be performed for the production of transgenic pigs.

키워드

Porcine Whey Acid Protein (WAP) Knock-in vector Tissue plasminogen activator (tPA) therapeutic proteins

저자

  • Ji-Youn Kim [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-Gun 55365, Korea ]
  • Mi-Ryung Park [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-Gun 55365, Korea ]
  • Hak-Jae Chung [ Swine Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, Korea ]
  • SungJune Byun [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-Gun 55365, Korea ]
  • KeonBong Oh [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-Gun 55365, Korea ]
  • Hyeon Yang [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-Gun 55365, Korea ]
  • Dong Hoon Kim [ Planning and Coordination Division, National Institute of Animal Science, Rural Development Administration, Wanju-Gun 55365, Korea ]
  • Kyung-Woon Kim [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-Gun 55365, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 The 17th International Symposium on Developmental Biotechnology

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.