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Poster Presentation : Sperm / Male Reproduction

Over Expression of Aminopeptidase N has Negative Effect on Boar Fertility

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.132-132
  • 저자
    Won-Ki Pang, Kyu-Ho Kang, Ki-Uk Kim, Ki-Jin Kwon, Sae-Han Kang, Amena Khatun, Do-Yeal Ryu, Md Saidur Rahman, Myung-Geol Pang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347390

원문정보

초록

영어
Artificial insemination is a commonly used technology in the porcine industry that directly linked to the genetic upgradation of future progeny and profitable farm management. Therefore, accurate prediction of boar fertility is a matter of paramount importance. Although prediction of semen/sperm fertility using conventional analysis provides a preliminary assumption of male fertility, its practical value is limited. Protein content in the cell provides an important and fascinating insight to the dynamics of cell function. Therefore, the study of protein function in spermatozoa and their corresponding relationship with male fertility might be an excellent alternative tool for more accurate prediction of male fertility. Recently we demonstrated that aminopeptidase N (APN) activity is negatively correlated with motility and fertility of mice spermatozoa. Here, we investigated whether APN is also correlated with the fertility of boar. Spermatozoa were collected from high and low fertility boar based on their field fertility data (average litter size 13.2±0.06 and 11.1±0.14, respectively). Simultaneously, APN levels were examined by Western blotting using the corresponding antibody. In addition, spermatozoa from both fertility groups were examined by conventional semen analysis such as computer assisted sperm analysis (CASA) and chlorotetracycline (CTC) staining. Our data showed a significant higher APN expression in low fertility boar spermatozoa compare to the fertile counterpart (p<0.05). Interestingly, no significant difference was noticed in motility, motion kinematics, and capacitation status between both groups. Based on this findings, it is tempting to speculate that APN activity might directly correlate with boar fertility. Therefore, APN activity in spermatozoa is more accurate and sensitive biomarker for the detection of boar fertility then conventional semen analysis. However, further studies and needed to confirm our initial findings

키워드

Aminopeptidase N (APN) Capacitation Porcine Spermatozoa Litter size

저자

  • Won-Ki Pang [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
  • Kyu-Ho Kang [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
  • Ki-Uk Kim [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
  • Ki-Jin Kwon [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
  • Sae-Han Kang [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
  • Amena Khatun [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
  • Do-Yeal Ryu [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
  • Md Saidur Rahman [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
  • Myung-Geol Pang [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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