Earticle

초록

영어

Sperm decondensation must be accomplished before fluorescence in situ hybridization (FISH). However, the lack of studies were conducted to discover the optimal porcine sperm decondensation for improving efficiency of FISH. We therefore sought to investigate the proper conditions of porcine sperm decondensation for FISH. In this trial, semen samples were obtained from eleven boar and immediately washed. After washing, the suspension was smeared and fixed on slides. Air-dried slides were incubated at 37℃ for 0, 15, 30 and 45 min in a 5 mmol/L dithiothreitol (DTT) to cleave protamine disulfide bonds, and thus sperm head decondensation was occurred. Subsequently, slides were counter-stained with hoechst 33258 to measure several parameters of sperm head by using microscope. The area, perimeter, and length of sperm head were significantly increased after 30 min incubation. This result demonstrated that sperm head is effectively expanded, which is essential for sperm decondensation after 30 min incubation. Subsequently, the roundness of sperm head was significantly increased after 15 min incubation. This result showed that the circularity of sperm head, which is important for sperm head decondensation, is ideal after 15 min. Taken together, the incubation time of optimal sperm head decondensation could be 30 min to get better achievement for FISH.

키워드

저자

• Ki-Jin Kwo [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Won-Ki Pang [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Kyu-Ho Kang [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Ki-Uk Kim [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Sae-Han Kang [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Dong-Ha Shin [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Amena Khatun [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Do-Yeal Ryu [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Md Saidur Rahman [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Myung-Geol Pang [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636