Earticle

초록

영어

Bisphenol A (BPA) is an industrial chemical widely used to manufacture certain plastics. As a strong endocrine disruptor, BPA is capable of altering cell functions. Exposure to BPA correlated with reduction of motility and fertilization ability of spermatozoa by increasing oxidative stress. Since antioxidants are capable to protect cells from oxidative damage, it may minimize BPA-induced stress to cells. Here, we examine whether exposure of spermatozoa to antioxidants such as glutathione (GSH), vitamin C (Vit C), and vitamin E (Vit E) has any protective effects of BPA-exposed spermatozoa. In this in vitro trials, collected spermatozoa from sexually mature male mice were incubated with BPA and different antioxidants (Control, BPA, BPA+GSH, BPA+Vit C, BPA+Vit E, GSH, Vit C, Vit E) for 6 hours. The doses of BPA and antioxidants were chosen based on preliminary studies (BPA: 100 μM, GSH: 5 mM, Vit C: 100 μM, and Vit E: 2 mM). Following incubation, we detect the levels of reactive oxygen species (ROS) in spermatozoa as a measure of oxidative stress to the cell. Our results showed that antioxidants are capable to protect oxidative stress by ROS in BPA-exposed spermatozoa (p<0.05). Simultaneously, we examine motility parameters and capacitation status of spermatozoa by CASA, and CTC staining, respectively. Strikingly, antioxidants treatment of BPA-exposed spermatozoa was capable to restore sperm motility (p<0.05). However, hyperactivated sperm motility, motion kinematics parameters, and capacitation status of spermatozoa showed no difference between treatments groups. This findings evidence that antioxidants are capable to restore sperm functions from BPA mediated toxicity. Therefore, antioxidants can be considered as therapeutic agents to treat BPA toxicity. However, further studies are needed to confirm our preliminary findings.

키워드

저자

• Kyu-Ho Kang [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Ki-Uk Kim [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Won-Ki Pang [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Ki-Jin Kwon [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Sae-Han Kang [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Amena Khatun [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Do-Yeol Ryu [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Md Saidur Rahman [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]
• Myung-Geol Pang [ Department of Animal Science and Technology, Laboratory of Reproductive Physiology and Modulation, Chung-Ang University, Gyeonggi-Do 17546, Republic of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636