Earticle

현재 위치 Home

Poster Presentation : Sperm / Male Reproduction

Comparison of Sperm Fertility and In Vitro Developmental Potential of Jeju Black Cattle Bull and Hanwoo Bull

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.127-127
  • 저자
    Jae-Wook Yoon, Sang-Gi Jeong, Min-Jee Park, Eun-Young Kim, Se-Pill Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347385

원문정보

초록

영어
The Jeju Black Cattle (JBC) are a type of traditional Korean native cattle with a characteristic black fur that covers the entire body. Jeju black cattle are smaller than other breeds and their disposition is very calm. The breed's qualities and genetic uniqueness helped it to be named as a protected, state-designated national monument in 2013. However, they are endangered crisis. Therefore, in vitro fertilization (IVF) technology is needed to preserve these endangered animals and to further improve their traits. In this study, we compared the sperm fertility and developmental potential of IVF embryos using Hanwoo sperm supplied by Korea Animal Improvement Association and JBC sperm made by Jeju Special Self-Governing Province Livestock bull semen Promotion Institute. [Hanwoo A, B (KPN) grade and JBC A, B two type]. Sperm was inseminated in 44 μL IVF drop contained 10 oocytes with sperm concentration of 1× 106 cells/mL, and then 2 μL heparin and 2 μL PHE (20 μM penicillamine, 10 μM hypotaurine, 2 μM epinephrine) were added. At 2 days after IVF, the fertilization rate was higher in Hanwoo sperm group (80.9±1.6) than in JBC sperm group (72.0±5.2). The IVF rate was significantly higher in Hanwoo-B sperm group (82.7±1.1) and JBC-B sperm group (84.5) than in JBC-A sperm group (67.0±5.1)(p<0.05). The blastocyst formation rate was the highest in Hanwoo-B sperm group (43.0±3.9) among four treatment sperm groups(p<0.05). The trophectoderm cell number was higher in Hanwoo sperm group (122±7.8) than in JBC sperm group (73±12.6). The inner cell mass cell number of Hanwoo sperm group (46±2.5) was similar to that of JBC sperm group (46±4.0). Thus, the total cell number of day 8 blastocyst was higher in Hanwoo sperm group (168±8.9) than in JBC sperm group (119±15.9). These results demonstrate that there were different in sperm quality (viability and motility) between Hanwoo sperm group (70∼80%) and JBC sperm group (60∼70%), and their in vitro development capacity was also presented as difference. We need to more sperm fertility study using JBC sperm.

키워드

Bovine Sperm Sperm fertility In vitro developmental

저자

  • Jae-Wook Yoon [ Jeju National University, Stem Cell Research Center, Jeju National University ]
  • Sang-Gi Jeong [ Jeju National University, Stem Cell Research Center, Jeju National University ]
  • Min-Jee Park [ Jeju National University, Stem Cell Research Center, Jeju National University, Mirae Cell Bio ]
  • Eun-Young Kim [ Jeju National University, Stem Cell Research Center, Jeju National University, Mirae Cell Bio ]
  • Se-Pill Park [ Jeju National University, Stem Cell Research Center, Jeju National University, Mirae Cell Bio ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 The 17th International Symposium on Developmental Biotechnology

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.