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Poster Presentation : Reprogramming / Epigenetic

Reprogramming using by mRNAs in Primary Somatic Cells derived from Patients with Brain Cancer

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.120-121
  • 저자
    Kyoung-Ha So, Young Seok Park, Sang-Hwan Hyun
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347382

원문정보

초록

영어
The ability to generate patient’s induced pluripotent stem cells (iPSCs) could provide tremendous promises for regenerative medicine. It has reported that choice of reprogramming tools is essential for the safety and the increasing efficiency of reprogramming process and transduction. Non-integrating techniques as like mRNA based reprogramming protocols are available for the production of genetically stable iPSCs while avoiding the risks of genomic integration. In this study, to derive brain cancer patient’s iPSCs by mRNA’s reprogramming, we obtained patient’s somatic tissues at brain tumor surgery that informed consent obtained according to institutionally- approved protocols. Patient's primary somatic cells were isolated, and they used as the cell source for iPSCs generation under xeno-free conditions. Reprogramming produced by non-integration methods utilized the combination of reprogramming mRNAs (OSKMNL) with evasion mRNAs (EKB) (Stemgent) during four days. Reprogrammed cells showed the colonies like iPSCs after six days of transduction, and the colonies were expanded until pick-up at day 14. These colonies were stained for pluripotency-associated genes using TRA-1-60 and TRA-1-81 by immunocytochemistry. Established colonies were also expanded without feeder condition and stained with Alkaline Phosphatase. In the further study, generated patient's iPSCs will be required to investigate their availability of the clinical application under xeno-free conditions which could provide a path to GMP applicability that should facilitate the clinical implementation of patient- or disease-specific iPSCs therapies.

키워드

Reprogramming Brain cancer Primary somatic cells iPSCs mRNA Xeno-free

저자

  • Kyoung-Ha So [ Institute for Stem Cell & Regenerative Medicine (ISCRM) and Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea, ]
  • Young Seok Park [ Department of Neurosurgery, Chungbuk National University Hospital, Chungbuk National University, College of Medicine, Cheongju 28644, Republic of Korea ]
  • Sang-Hwan Hyun [ Institute for Stem Cell & Regenerative Medicine (ISCRM) and Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea, ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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