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Poster Presentation : Reprogramming / Epigenetic

Derivation of Canine induced Pluripotent Stem Cells using an Integration-Free Method

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.118-119
  • 저자
    Mirae Kim, Seon-Ung Hwang, Sang-Hwan Hyun
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347381

원문정보

초록

영어
Canine induced Pluripotent Stem Cells (iPSCs) can provide great potential for regenerative veterinary medicine and may assist in the development of new therapeutics and pre-clinical studies for both dogs and human. To date, there have been several reports on the generation of canine iPSCs using retroviral or lentiviral transduction of Yamanaka’s factors. However, there is no report of canine iPSCs generated by genomic integration-free methods. According to previous studies, a polycistronic and synthetic self-replicating RNA system was developed for generating human iPSCs by the RNA replicon of Venezuelan Equine Encephalitis (VEE) virus. The VEE replicon is a positive-sense and single-stranded RNA which is similar with cellular mRNA containing a 5’ cap and poly (A) tail. It is no potential for genomic DNA integration problems because it does not use a DNA intermediate. Here, we investigated to generate canine iPSCs by a single transfection of the VEE-reprogramming factor (VEE-RF) RNA. To generate integration- free canine iPSCs, the VEE-OKS-iG RNA that expresses four reprogramming ORFs (hOct4, hKlf4, hSox2 and hGlis1) was transfected. Also, B18R mRNA was co-transfected to reduce immune response by VEE replicon. Putative canine iPSC colonies first appeared between day 15-25. Interestingly, they have two distinct types of initial canine iPSC colonies. They were identified by immunohistochemistry of live cells using TRA-1-60 antibody and also showed clear alkaline phosphatase (AP) activity. Further study will be required to analyze the characterization for a clinical application, and the non-integrating and self-replicating VEE RNA replicon system has the potential to make a great contribution to generating clinically applicable canine iPSCs.

키워드

Canine induced Pluripotent stem cells Integration-free Reprogramming VEE (Venezuelan Equine Encephalitis) virus

저자

  • Mirae Kim [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea ]
  • Seon-Ung Hwang [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea ]
  • Sang-Hwan Hyun [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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