Earticle

초록

영어

The naked mole rat (NMR, Heterocephalus glaber) is an emerging model animal for researching on aging and cancer due to their exceptionally long life span and cancer-resistance. It has been proven that carcinogenesis and obtaining pluripotency from normal cells undergo similar cellular and molecular mechanisms. Here, we tried to reprogram fibroblasts for investigating cellular reprogramming process in NMR having anti-cancer property. Firstly, we investigated which culture conditions and reprogramming factors were adequate for reprogramming of NMR cells. Mouse reprogramming factors and high glucose culture conditions efficiently facilitated pluripotent genes of NMR fibroblasts. However, as tumor-repressor genes such as NF2 and Rb1 were highly expressed, expression of pluripotent genes were gradually decreased during reprogramming, and no primary colonies of iPSCs were derived. Next, tumor suppressor genes were inhibited by using RNA interference technique to improve reprogramming efficiency. As a result, HAS2-knocked down cells were converted into AP-positive iPSCs by ectopic expression of reprogramming factors, and NMR iPSCs could be differentiated into beating cardiomyocytes and endodermal cells. Overall, we derived NMR iPSCs through downregulating tumor suppression mechanism, which could be applied for researches of ageing and cancer.

키워드

저자

• Kwang-Hwan Choi [ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, Korea ]
• Sang-Goo Lee [ Division of Genetics, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA ]
• Dong-Kyung Lee [ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, Korea ]
• Jong-Nam Oh [ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, Korea ]
• Seung-Hun Kim [ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, Korea ]
• Tae-Young Park [ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, Korea ]
• Min-Gyun Lee [ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, Korea ]
• Man Ho Cho [ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, Korea ]
• Chang-Kyu Lee [ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Institute of Green Bio Science and Technology, Seoul National University, Pyeong Chang, Kangwon do, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636