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Poster Presentation : Oocyte Maturation / Embryonic Development

Evaluation the Fertilization of Porcine In Vitro Matured Oocyte using by Ex Vivo Model

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.112-112
  • 저자
    Yongquan Han, Dibyendu Biswas, Sang-Hwan Hyun
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347377

원문정보

초록

영어
Porcine uterine ex vivo model is a useful tool for investigating gamete maturation, activation, fertilization and early embryonic development. This study was to evaluate fertilization ability of in vitro matured (IVM) porcine oocyte (MII) using porcine uterine ex vivo model. IVM-MII oocytes were fertilized with 7.5×107, 15×107 and 30×107 sperm for 20min in porcine uterine ex vivo model. And, the oocytes were then flushed and performed in vitro culture (IVC). IVM-MII oocytes used for in vitro fertilization (IVF) served as control 1. Before IVF, IVM-MII oocytes cultured in porcine uterine ex vivo model for 20min served as control 2. In the results, it was found that the sperm penetration rate in the control 2 (73.0±4.2) was significantly higher than the other treatment group: 7.5×107 (29.7±4.4), 15×107 (34.3±3.2) and 30×107 (44.3±7.4). But there was no significant difference between control 1 and control 2. Monospermic penetration rate in all treatment groups was significantly higher than control groups, and polyspermy rate in all treatment group was also significantly lower than control groups. These results suggested that porcine uterine ex vivo model improved monospermic penetration by reducing sperm penetration ratio. In the further study, it is required to evaluate the embryonic development, GSH and ROS accumulation levels of MII oocytes fertilized using porcine uterine ex vivo model.

저자

  • Yongquan Han [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea ]
  • Dibyendu Biswas [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea ]
  • Sang-Hwan Hyun [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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