The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
페이지
pp.112-112
저자
Yongquan Han, Dibyendu Biswas, Sang-Hwan Hyun
언어
영어(ENG)
URL
https://www.earticle.net/Article/A347377
원문정보
초록
영어
Porcine uterine ex vivo model is a useful tool for investigating gamete maturation, activation, fertilization and early embryonic development. This study was to evaluate fertilization ability of in vitro matured (IVM) porcine oocyte (MII) using porcine uterine ex vivo model. IVM-MII oocytes were fertilized with 7.5×107, 15×107 and 30×107 sperm for 20min in porcine uterine ex vivo model. And, the oocytes were then flushed and performed in vitro culture (IVC). IVM-MII oocytes used for in vitro fertilization (IVF) served as control 1. Before IVF, IVM-MII oocytes cultured in porcine uterine ex vivo model for 20min served as control 2. In the results, it was found that the sperm penetration rate in the control 2 (73.0±4.2) was significantly higher than the other treatment group: 7.5×107 (29.7±4.4), 15×107 (34.3±3.2) and 30×107 (44.3±7.4). But there was no significant difference between control 1 and control 2. Monospermic penetration rate in all treatment groups was significantly higher than control groups, and polyspermy rate in all treatment group was also significantly lower than control groups. These results suggested that porcine uterine ex vivo model improved monospermic penetration by reducing sperm penetration ratio. In the further study, it is required to evaluate the embryonic development, GSH and ROS accumulation levels of MII oocytes fertilized using porcine uterine ex vivo model.
Yongquan Han [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea ]
Dibyendu Biswas [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea ]
Sang-Hwan Hyun [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea ]