Earticle

현재 위치 Home

Poster Presentation : Oocyte Maturation / Embryonic Development

Supplement of GDF8 during In Vitro Culture of Porcine In Vitro Fertilized Embryo improved Embryonic Developmental Competence

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.106-107
  • 저자
    Junchul David Yoon, Eunsong Lee, Sang-Hwan Hyun
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347374

원문정보

초록

영어
Growth differentiation factor8 (GDF8) is a member of the transforming growth factor-β that has been identified as a robust physiological regulator. According to recent studies, the GDF8 is detected in oviduct fluid and uterus which led us to suggest that the GDF8 may effect on preimplantation embryonic development and act paracrine role to correlate with successful late-blastocyst implantation in in vivo. We investigated the effect of GDF8 supplement during in vitro culture (IVC) of porcine embryos derived from in vitro fertilization (IVF) on cleavage and blastocyst (BL) formation rate, and gene transcription level analysis in BL. Data were analyzed by one way ANOVA, followed by Tukey’s range test. Respectively 0.2, 2 and 20 ng/mL of GDF8 were added during IVC, and experimental groups were as follows; control (0), 0.2, 2, and 20 GDF8 supplement groups. At IVC 48hr, there was no significant difference on cleavage rate from the different concentration of GDF8 supplement groups (65.7%, 66.0%, 66.3%, and 65.8%, respectively). After additional 120hr of embryo culture, 0.2 group was shown significantly (p<0.05) higher than control in blastocyst formation rate and total cell number (32.5% and 88.0±7.3 VS 40.4% and 118.4±12.7, respectively). Using the achieved IVF BLs, the specific gene expression pattern were evaluated. The embryo development competence marker Pcna, Pou5f1 and Sox2 (1.81, 2.85 and 2.09 times, respectively), and cell junction assembly regulator Adam10, Adam17, Tjp1, Cdh1 (1.40, 1.98, 1.79 and 1.80 times, respectively) genes mRNA transcript levels in 0.2 group were significantly increased in 0.2 group compared with control. Moreover, pro-apoptotic factor Cas3 gene mRNA transcript levels was significantly decreased in 0.2 group than control (0.62 times). In conclusion, the supplementation of 0.2 ng/mL GDF8 during IVC significantly improved embryonic developmental potential via regulating embryo developmental competence markers and cell junction assembly regulator transcriptional levels.

키워드

Porcine Embryo In vitro culture GDF-8 In vitro fertilization

저자

  • Junchul David Yoon [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of Korea ]
  • Eunsong Lee [ Laboratory of Theriogenology, College of Veterinary Medicine, Kangwon National University, Kangwon, Republic of Korea ]
  • Sang-Hwan Hyun [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 The 17th International Symposium on Developmental Biotechnology

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.