The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
페이지
pp.105-105
저자
Yeong-Hun Kang, Sang-Hwan Hyun
언어
영어(ENG)
URL
https://www.earticle.net/Article/A347373
원문정보
초록
영어
Alpha lipoic acid (ALA) is a biological membranes compound. as the antioxidant, it decreases the oxidized forms of other antioxidant substances such as vitamin C, vitamin E and glutathione (GSH). To examine the effect of ALA on the in vitro maturation (IVM) of porcine oocytes, we investigated intracellular GSH and reactive oxygen species (ROS) levels, and subsequent embryonic development after parthenogenetic activation (PA). Intracellular GSH levels in oocytes treated with 50uM of ALA increased significantly (p<0.05) and exhibited a significant (p<0.05) decrease in intracellular ROS levels compared with the control group. Oocytes matured with 50uM during IVM displayed significantly higher cleavage rates (67.8% vs. 83.4%, respectively), and higher blastocyst formation rates and total cell number of blastocysts after PA (31.6%, 58.49 vs. 46.8%, 68.58, respectively) than the control group. In conclusion, these results suggest that treatment with ALA during IVM improves the nuclear maturation of porcine oocytes by increasing the intracellular GSH levels, thereby decreasing the intracellular ROS levels and subsequent embryonic developmental potential of PA.
Yeong-Hun Kang [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea ]
Sang-Hwan Hyun [ Institute for Stem Cell & Regenerative Medicine (ISCRM), Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea ]