Earticle

초록

영어

Bisphenol A (BPA) is an environmental contaminant widely used in the plastic industry. BPA has been demonstrated to be an endocrine disruptor and has an adverse effect on the embryonic development of mammals. However, the mechanism of action of BPA is limited. In this study, we investigated the role and mechanism of BPA in porcine embryonic development. First, the parthenotes were treated with different concentrations of BPA. We found that blastocyst formation was impaired and the parthenotes were arrested at the 4-cell stage after treatment with 100 μm BPA. Second, ROS increased following the addition of BPA, which further caused mitochondrial damage, and cytochrome c was released from the mitochondria to induce apoptosis. The adaptive response was demonstrated through LC3 immunofluorescence staining and by assessing autophagy-related gene expression. In addition, BPA caused DNA damage through the p53-p21 signaling pathway. Thus, our results indicate that BPA displays an adverse effect on porcine early embryonic development through mitochondrial and DNA damage.

키워드

저자

• Kyung-Tae Shin [ Department of Animal Sciences, Chungbuk National University, Cheongju, Korea ]
• Jing Guo [ Department of Animal Sciences, Chungbuk National University, Cheongju, Korea ]
• Yong-Dae Ahn [ Cheongwon Natural Island, 203, Urongni, Seowongu, Cheongju 362-823, Korea ]
• Dong-Jin Juang [ Department of Animal Sciences, Chungbuk National University, Cheongju, Korea ]
• Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University, Cheongju, Korea ]
• Xiang-Shun Cui [ Department of Animal Sciences, Chungbuk National University, Cheongju, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636