Earticle

현재 위치 Home

Poster Presentation : Oocyte Maturation / Embryonic Development

Zinc Controls the Transition from Asymmetric to Symmetric Cell Division in Mouse Oocytes and Zygote Maturation Via the Actin Nucleator Spire

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.100-100
  • 저자
    Yu-Jin Jo, In-Won Lee, Seung-Min Jung, Jeongwoo Kwon, Suk Namgoong, Nam-Hyung Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347368

원문정보

초록

영어
Zinc plays an essential role in mammalian oocyte maturation, fertilization, and early embryogenesis. The zinc concentration increases during oocyte maturation, but decreases soon after fertilization. Depletion of zinc impairs various stages of oocyte maturation, including cell cycle control, asymmetric division, and cytokinesis. However, the exact mechanisms by which zinc is involved in these processes is unknown. Here, we report that zinc, via the actin nucleator Spire, acts as an essential regulator of the actin cytoskeleton remodeling during mouse oocyte maturation and fertilization. Depletion of zinc in the mouse oocyte by either chemical chelation or knockdown of zinc transporters impaired cortical and cytoplasmic actin formation. Spire colocalized with zinccontaining vesicles via its FYVE domain, which contains zinc finger motifs that are essential for proper localization: mutation of zinc-binding cysteines in the FYVE domain abolished proper localization and actin mesh formation in oocytes. Expression of Spire truncation mutants revealed that all three major domains of the actin nucleator are required for correct localization and activity. After fertilization/parthenogenetic activation, Spire localization was dramatically altered following zinc release from the oocyte. Collectively, our data reveal novel roles for zinc in the regulation of the actin nucleator Spire and suggest a novel mechanism for the transition from asymmetric to symmetric cell division during mammalian oocyte maturation and fertilization.

키워드

Oocyte maturation Actin filament Actin nucleators

저자

  • Yu-Jin Jo [ Department of Animal Science, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Cheongju 361-763, Republic of Korea ]
  • In-Won Lee [ Department of Animal Science, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Cheongju 361-763, Republic of Korea ]
  • Seung-Min Jung [ Department of Animal Science, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Cheongju 361-763, Republic of Korea ]
  • Jeongwoo Kwon [ Department of Animal Science, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Cheongju 361-763, Republic of Korea ]
  • Suk Namgoong [ Department of Animal Science, Chungbuk National University, Cheongju 361-763, Republic of Korea ]
  • Nam-Hyung Kim [ Department of Animal Science, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Cheongju 361-763, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 The 17th International Symposium on Developmental Biotechnology

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.