Earticle

초록

영어

LIM kinase(LIMK)s which includes LIMK1 and LIMK2, belongs to serine kinases and is discovered more than a decade ago, and LIMK is shown to be a regulator of actin dynamics. LIMKi3 is a specific inhibitor of LIMK including LIMK1 and LIMK2. Previous results show that LIMKi3 suppresses porcine oocyte maturation in vitro by affecting of actin distribution. However, the roles of LIMKi3 in porcine in early embryo stage embryos is unknown. In this study, we investigated the role of LIMK and LIMK inhibitor function in actin filament organization by affecting p-cofilin, And, we also investigated the cell junction assembly during early embryo development. Firstly, we confirmed LIMK and p-cofilin localization by immunocytochemistry and confocal microscopy. LIMK localized in cytoplasm and cell junction with F-actin after morula. p-Cofilin localized in cytoplasm during early embryo development. LIMKi3(10 uM) treatment with LIMKi3 after parthenogenetic activation, blastocyst rates was significantly decreased compared to the control group. After LIMKi3 treatment in morula, intensity levels of actin and p-cofilin, a downstream molecule of LIMK, were decreased in morula. Furthermore, cell junction related proteins were significantly decreased in LIMKi treatment. Thus, our result indicate that Limk interferes with porcine early embryo development and reduces actin formation by affecting cell junction assembly.

키워드

저자

• Min-Jung Seong [ Department of Animal Sciences, Chungbuk National University ]
• Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636