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Poster Presentation : Oocyte Maturation / Embryonic Development

2i System Enhances Porcine Blastocyst Quality by Regulating of Epigenetic Modification

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.96-96
  • 저자
    Jeongwoo Kwon, XingHui Shen, Jiang Hao, Yao Xuerui, Nam-Hyung Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347364

원문정보

초록

영어
2i system is useful for maintenance of navie state of embryonic stem cells in various species. However, the role of 2i system in porcine preimplantation stage embryos unclear. In this study, we investigated the quality of blastocyst and pluripotent-related factor by 2i system. And, we also investigated about DNA and RNA epigenetic modification status by 2i system during porcine early embryo development. After treatment of MEK1/2 inhibitor PD0325901(4 μM), GSK-3 inhibitor CHIR99021(0.3 μM) and 2i (PD0325901+CHIR99021) during pre-implantation development, blastocyst formation rate had no significantly difference. However, blastocyst size increased in 2i treatment embryos. Blastocyst quality also increased after treatment of CHIR and 2i using TUNEL assay and Brdu assay. Interestingly, main transcription factor OCT4 and SOX2 positive cells in blastocyst was significantly increased in 2i groups. Especially, histone modification- relation proteins like h3k9me3 and h3k9ac changed after treatment of CHIR and 2i. Global gene expression patterns related with pluripotency and epigenetic also changed in inhibitor treatment groups. Furthermore, bi-sulfite sequencing results showed that 2i treatment groups demethylated in satellite I region. In conclusion, our findings strongly suggest that 2i system can be useful method for increasing of blastocyst quality by affecting of ICM/TE formation by regulating epigenetic modification during early embryo development.

키워드

2i system Early embryo Pluripotency Epigenetic modification

저자

  • Jeongwoo Kwon [ Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea ]
  • XingHui Shen [ Department of Animal Sciences, Chungbuk National University, Department of Histology and Embryology, Harbin Medical University, Harbin, China ]
  • Jiang Hao [ Department of Animal Sciences, Chungbuk National University, Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, 130062, China ]
  • Yao Xuerui [ Department of Animal Sciences, Chungbuk National University, Department of Veterinary Medicine, College of Agriculture, Yanbian University, Yanji, Jilin Province, China ]
  • Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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