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Poster Presentation : Oocyte Maturation / Embryonic Development

Cobalamin Supplementation during In Vitro Maturation Improves Developmental Competence of Porcine Oocytes

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.94-94
  • 저자
    Pantu Kumar Roy, Xun Fang, Bahia MS Hassan, Sang Tae Shin, Jong Ki Cho
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347362

원문정보

초록

영어
The objective of this experiment was to explore the effects of cobalamin prior to in vitro maturation (IVM) of immature pig oocyte. Specifically, the effects of cobalamin exposure on nuclear and cytoplasmic maturation, diameter, intracellular glutathione (GSH) and reactive oxygen species (ROS), and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT), and gene expression levels in SCNT embryos have been measured. Cumulus oocyte complexes (COCs) have been exposed in 200 pM of cobalamin for 22 h, and afterward cultured for 22 h without exposure of cobalamin. The COCs that were matured in the same IVM for 44 h without cobalamin used as control group. Diameter of cobalamin treated oocytes were significantly greater than control group. GSH was higher in cobalamin group than control oocytes group but reduction of ROS in cobalamin than control group of oocytes. In PA, exposure with cobalamin has been significantly greater than control group in rates of maturation, cleavage and blastocyst formation. Similarly, in SCNT embryos rates of maturation, cleavage and formation of blastocyst have been also significantly greater in cobalamin group than control group. SCNT embryos treated with cobalamin 22 h in IVM showed greater expression levels of POU5F1, DPPA2 and NDP52Il mRNA compared with control group. Our results demonstrate that cobalamin treatment prior to IVM improves the development competence of porcine oocyte.

키워드

Cobalamin Development Embryo Porcine ROS

저자

  • Pantu Kumar Roy [ Lab. of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea ]
  • Xun Fang [ Lab. of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea ]
  • Bahia MS Hassan [ Lab. of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea ]
  • Sang Tae Shin [ Lab. of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea ]
  • Jong Ki Cho [ Lab. of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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