Earticle

초록

영어

OPU (Ovum pick up) has been an important technique to increase genetic resources for early establishment of genetically important herd and in vitro embryo production system. This study compared relationship between OPU-derived COCs quality and embryo development. The recovery procedures of COCs were performed twice a week using ultrasound guided OPU tools. The 18 gauge disposable needle was connected into 50 mL conical tube by teflon tubing, The grade of COCs was evaluated by microscopic examination. Grade A had 3 or more layers of cumulus cell and compact cytoplasm. Grade B had 1~3 layers of cumulus cell and compact cytoplasm. Grade C had 1 layers cumulus cell and compact cytoplasm. Grade D was denuded oocyte and poor cytoplasm. The collected COCs were fertilized in vitro and developed for 7~8 day in mSOF media. The cleavage rate of grade A/B dominant pick-up group (above 60% of A/B grade rate from total recovered COCs) was 48.9% and that of poor quality was 51.9%. The rate of blastocyst rate in grade A/B dominant OPU procedures after IVF was 28.9%, but poor quality group (below 60% of A/B grade COCs) was 14.8%. The developed blastocysts were transferred into recipient or cryopreserved for further research. When total 11 blastocysts were transferred into surrogate cows with the pregnancy rate (4/11) of 36.3% and 4 calves are expected.

키워드

저자

• Dongkyo Kim [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]
• Namtae Kim [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]
• Sung Woo Kim [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]
• Chan-lan Kim [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]
• Min Su Kim [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]
• Ik Soo Jeon [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636