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Poster Presentation : Oocyte Maturation / Embryonic Development

Antioxidant Effect of Alpha-Linolenic Acid during In Vitro Maturation in Porcine Oocytes

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.88-88
  • 저자
    Ji-Eun Lee, Yong Hwangbo, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347357

원문정보

초록

영어
Alpha-linolenic acid (ALA) is one of n-3 polyunsaturated fatty acids and found mainly in the chloroplasts. Many studies have been reported that when intracellular reactive oxygen species (ROS) in mammalian oocytes was reduced by supplementation of ALA in in vitro maturation (IVM) medium. Based on these reports, we expected that ALA acts as an antioxidant during IVM of porcine oocytes. Therefore, the objective of this study was to investigate the antioxidant effect of ALA supplementation during IVM in porcine oocytes. The cumulus-oocyte complexes (COCs) were incubated in IVM medium containing 200 μM H2O2 or H2O2 with 50 μM ALA for 44 h. Nuclear maturation stage of oocytes was evaluated using aceto-orcein method. For measurement of oxidative stress status, intracellular ROS and glutathione (GSH) levels were measured using carboxy-DCFDA and cell tracker red, respectively. In results, oocytes in metaphase-II stage were reduced by H2O2 treatment (control: 80.00%; H2O2 grop: 61.84%) and it was slightly recovered by treatment of ALA (69.76%) (p<0.05). The intracellular GSH levels decreased in H2O2 groups compared with control groups, but it was enhanced by ALA treatment (p<0.05). On the contrary, H2O2 treatment increased intracellular ROS level in oocytes and H2O2 induced ROS was decreased by treatment of ALA (p<0.05). Our findings shown that ALA treatment under oxidative stress environment recovered oocyte maturation via increase of GSH and decrease of ROS in oocytes. Therefore, these results suggest that ALA have an antioxidative ability and it could be used as antioxidant in in vitro production system of porcine embryo.

키워드

Alpha-linolenic acid In vitro maturation Porcine oocyte Reactive oxygen species Glutathione

저자

  • Ji-Eun Lee [ College of Animal Life Science and Kangwon National University, Chuncheon 24341, Republic of Korea ]
  • Yong Hwangbo [ College of Animal Life Science and Kangwon National University, Chuncheon 24341, Republic of Korea ]
  • Hee-Tae Cheong [ College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea ]
  • Boo-Keun Yang [ College of Animal Life Science and Kangwon National University, Chuncheon 24341, Republic of Korea ]
  • Choon-Keun Park [ College of Animal Life Science and Kangwon National University, Chuncheon 24341, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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