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Poster Presentation : Oocyte Maturation / Embryonic Development

Effects of γ-Tocotrienol and L-Ascorbic Acid on In Vitro Maturation and Developmental Competence under the Oxidative Stress Condition in Porcine Oocytes

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.87-87
  • 저자
    Hwa-Young Kim, Seung Tae Lee, Eun Song Lee, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347356

원문정보

초록

영어
The objective of this study was to investigate the effect of γ-tocotrienol (GTT; 50 μM) and/or L-ascorbic acid (Vc; 100 μM) under the oxidative stress condition (H2O2; 200 μM) on in vitro maturation and developmental competence in pigs. Porcine cumulus- oocyte complexes (COCs) were cultured in IVM medium containing GTT and/or Vc under the H2O2 condition for 44 h. After maturation, COCs were denuded and stained by orcein for evaluation of nucleic maturation. Intracellular reactive oxygen species (ROS) and glutathione (GSH) were respectively stained using carboxy-DCFDA and cell tracker red. Cleavage rate was determined in day 2 and day7, and blastocyst rate was determined in day 7 after in vitro fertilization. In results of nuclear maturation, oocytes in metaphase-Ⅰ (MI) were significantly higher in H2O2 treatment groups than GTT and/or Vc treatment groups (p<0.05). However, oocytes in MII stage were significantly increased in GTT treatment group than other treatment groups (p<0.05). Intracellular level of GSH was significantly increased by supplementation of GTT and/or Vc compared to control group (p<0.05). On the other hand, intracellular level of ROS was reduced in only GTT groups compared to other groups (p<0.05). In vitro development results showed that the cleavage rate of day 2, day 7 and blastocyst rate in GTT treatment group under the H2O2 condition were higher than other groups (p<0.05). Therefore, we suggest that treatment of GTT is effective on oocyte maturation and developmental competence rather than combined treatment of GTT and Vc in pigs.

키워드

Antioxidant In vitro maturation γ-Tocotrienol L-Ascorbic acid Porcine oocyte

저자

  • Hwa-Young Kim [ College of Animal Life Sciences and Kangwon National University, Chuncheon 24341, Korea ]
  • Seung Tae Lee [ College of Animal Life Sciences and Kangwon National University, Chuncheon 24341, Korea ]
  • Eun Song Lee [ College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
  • Choon-Keun Park [ College of Animal Life Sciences and Kangwon National University, Chuncheon 24341, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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