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Poster Presentation : Implantation / Uterine Biology

Class II Trans Activator and Major Histocompatibility Complex Class II Molecules: Expression at the Maternal-Conceptus Interface in Pigs

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.79-79
  • 저자
    Inkyu Yoo, Jisoo Han, Soogil Chae, Soohyung Lee, Hakhyun Ka
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347351

원문정보

초록

영어
For the successful pregnancy maternal immune response must be regulated to tolerate the semi-allogenic conceptus. In adaptive immunity, major histocompatibility complex class II (MHC class Ⅱ) molecules play critical role in presenting foreign antigens to the other immune cells. During early pregnancy in pigs, various types of endometrial cells, including epithelial cells, vascular endothelial cells, stromal cells, and infiltrated immune cells are activated by conceptus-derived interferon gamma (IFNG) and express immune regulatory molecules. Therefore, we determined the expression and regulation of MHC class II molecules, swine leukocyte antigen (SLA) -DM, -DO, -DR and -DQ and their transcription coactivator CIITA at the maternal-conceptus interface. We obtained endometrial tissue samples from day (D) 12 and D15 of the estrous cycle and D12, D15, D30, D60, D90 and D114 of pregnancy. Quantitative real-time PCR anaysis showed that mRNA levels of all genes dramatically increased on D15 of pregnancy. To determine the effect of interferon-gamma (IFNG) on SLAs and CIITA expression, we took advantage of explant culture and found that all of SLAs and CIITA were up-regulated by IFNG in a dose-dependent manner. Immunohistochemistry showed that CIITA protein was localized to vascular endothelial cells and stroma near the luminal epithelium on D15 of pregnancy. SLA-DQ protein is localized to stromal leukocytes and vascular endothelial cells during the estrous cycle and early pregnancy with the highest signal intensity on D15 of pregnancy. These results indicate that CIITA and MHC class II molecules are expressed in the uterine endometrium in a cell-type and stage-specific fashion during pregnancy, and may play a critical role in regulation of maternal immune response to support the establishment and maintenance of pregnancy at the maternal- conceptus interface in pigs.

키워드

Pig Endometrium IFNG CIITA MHC class II

저자

  • Inkyu Yoo [ Division of Biological Science and Technology, Yonsei University ]
  • Jisoo Han [ Division of Biological Science and Technology, Yonsei University ]
  • Soogil Chae [ Division of Biological Science and Technology, Yonsei University ]
  • Soohyung Lee [ Division of Biological Science and Technology, Yonsei University ]
  • Hakhyun Ka [ Division of Biological Science and Technology, Yonsei University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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