Earticle

현재 위치 Home

Poster Presentation : Implantation / Uterine Biology

Different Regulation of Plasminogen Activators and Their Inhibitor by Progesterone and 17β-Estradiol in Porcine Uterine Epithelial Cells

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.78-78
  • 저자
    Yong Hwangbo, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347350

원문정보

초록

영어
This present study was conducted to investigate different regulatory pattern of mRNA expression and activity of two-type plasminogen activators (PAs; urokinase-type, uPA; tissue-type. tPA), uPA specific receptor (uPAR), and type-1 PA inhibitor (PAI-1) by different concentration and exposure time of progesterone (P4) and 17β-estradiol (E2) in porcine uterine epithelial cells. Cultured epithelial cells were incubated with different concentrations of P4 or E2 (0, 0.1, 1, 5, 10, and 20 ng/mL) for 5 min or 24 h, respectively. In results, 24 h treatment of P4 or E2, and E2 treatment for 5 min did not affect to mRNA expression of uPA, tPA, uPAR, and PAI-1. In P4 treatment groups for 5min, the mRNA expression of uPA, uPAR and PAI-1 were increased in 20 ng/mL P4 groups compared with 0.1 ng/mL P4 treatment groups (p<0.05), whereas it is no different with control group (0 ng/mL). On the other hand, 10 and 20 ng/mL P4 treatments enhanced the mRNA expression of tPA compared with 0 ng/mL treatment in epithelial cells (p<0.05). When these cells were exposed to P4 or E2 for 5 min, only 0.1 ng/mL P4 increased PAs activity (p<0.05). Although mRNA expression of PAs were not influenced by exposure of sex hormone for 24 h, PAs activity was increased by 10 and 20 ng/mL treatments in both P4 and E2 (p<0.05). In conclusion, short term treatment of P4 upregulated uPA, tPA, uPAR. and PAI-1 mRNA expression, but E2 did not affect in both of short- and long-term treatment. Interestingly, PAs activity was increased by long term treatment of P4 and E2. These results show that mRNA expression of PAs are differently regulated by different concentration and exposure time of sex hormones, however, pattern of PAs activity is different with mRNA expression. Therefore, more studies regarding with regulatory mechanism of PA system are needed.

키워드

Pig Progesterone 17β-estradiol PAs system Uterine epithelial cells

저자

  • Yong Hwangbo [ College of Animal Life Sciences and Kangwon National University, Chuncheon 24341, Korea ]
  • Hee-Tae Cheong [ School of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
  • Boo-Keun Yang [ College of Animal Life Sciences and Kangwon National University, Chuncheon 24341, Korea ]
  • Choon-Keun Park [ College of Animal Life Sciences and Kangwon National University, Chuncheon 24341, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 The 17th International Symposium on Developmental Biotechnology

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.