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Poster Presentation : General Reproduction／Superovulation／AI／ET

Effect of Optixcell Extenders on Motility, Plasma Membrane Integrity, Acrosomal Membrane Integrity of Spermatozoa and Calving Rates following Artificial Insemination in Hanwoo

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.61-61
  • 저자
    Sung-Sik Kang, Sang-Rae Cho, Ui-Hyung Kim, Seok-dong Lee, Myung-Suk Lee, Byeong-Chul Yang, Boh-Suk Yang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347337

원문정보

초록

영어
Conventional semen extenders contained egg yolk as cryoprotectant. However, egg yolk is an animal protein and has a risk of zoonotic disease such as influeza virus. Thus, the demand of other chemically defined substitutes is required. As substitutes of egg yolk, liposomes are used in semen extenders. The structure of liposomes is already defined and manufacturing of liposome is possible. However, there are few reports of field trial on liposome based semen extender. Thus, we examined the effect of a liposome-based extender (Optixcell) on frozen- thawed spermatozoa characteristics and calving rate. Tris-citric egg-yolk extender (Triladyl) used as a control. Motility, motility parameters, plasma membrane integrity, acrosomal membrane integrity of spermatozoa and calving rate were compared. Percentages of total motile spermatozoa were similar in the Optixcell and Triladyl groups. However, among motile spermatozoa with straight line velocity (VSL) ≥ 25 μm/sec, the curvilinear velocity (VCL, μm/sec), VSL (μm/sec), average path velocity (VAP, μm/sec), amplitude of lateral head displacement (ALH, μm), beat cross frequency (BCF, Hz), and plasma membrane integrity of frozen-thawed spermatozoa of the Optixcell group were significantly higher than those of the Triladyl group. Furthermore, calving rate in the Optixcell group (79.0%) was higher than that of the Triladyl group (62.8%). However, acrosomal membrane integrity of frozen-thawed spermatozoa in the Optixcell and Triladyl groups was similar. These results indicate that semen freezing with Optixcell improved motility and plasma membrane integrity of frozen-thawed spermatozoa and calving rate of Hanwoo cows (native Korean cattle). In conclusion, our results suggest that semen freezing with the liposome-based extender Optixcell is more efficient than the tris-citric egg-yolk extender Triladyl for improvement of offspring production.

키워드

Calving rate Hanwoo Semen extender Sperm motility

저자

  • Sung-Sik Kang [ Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 25340, Republic of Korea ]
  • Sang-Rae Cho [ Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 25340, Republic of Korea ]
  • Ui-Hyung Kim [ Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 25340, Republic of Korea ]
  • Seok-dong Lee [ Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 25340, Republic of Korea ]
  • Myung-Suk Lee [ Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 25340, Republic of Korea ]
  • Byeong-Chul Yang [ Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 25340, Republic of Korea ]
  • Boh-Suk Yang [ Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 25340, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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