Earticle

초록

영어

The coat and muzzled colors in mammals are determined by the relative distribution of pheomelanin and eumelanin. In melanocytes, the expression of these two pigments is controlled by the melanocortin 1 receptor (MC1R) as well as by agouti locus alleles. For example, high expression of the AC (Adenylate cyclase 1) family genes, which interact with MC1R may affect the expression of CREB. leading to the over-expression of melanogenesis-associated genes such as PRKACB and CAMK2α. Further, increased expression of genes involved in tyrosine metabolism, such as TYR, is likely to affect the synthesis of eumelanin in the dark-muzzle cows. Our previous result, that the found intriguing distinction between the dark and light muzzle with respect to the expression of genes involved in the MAPK vs. Wnt signaling pathways. The aim of the present study was to identify melanogenesis-associated genes that are differentially expressed in the yellow and brindle coat of Korean native cattles. The first results, The expressed evaluation of MC1R protein were mostly highly between KBC to KDC, and there level at ST of KDC was higher than we expected, and decreased expressed in large sweat gland of epidermis zone. The RT-PCR analysis found that MC1R expressed at the KBC showed the same results like it was localization analyzed. Especially, levels of MC1R mRNA were elevated in HC compared to another group, while that the expression mostly higher in KDC. Next secondly results, was showed that the associated- genes of Want and MARK pathway were differential expression between KBC and KHC. Especially, in the KBC of malanogenesis major genes expression was highly, but the Want and minor genes were highly expression from KHC. In addition, analysis result of DNA methylation in Bos taurus genomes to survey the coat color–related epigenetic markers (differential methylated CpGs, DM CpGs) by RRBS was the appears that KBC has the biological functions of the DM CpG-associated genes. Regarding the increasing interest in the genetic diversity of cattle stocks, genes we identified for differential expression of melanogenesis and DM CpG-associated genes in the KBC vs. KHC may serve as novel markers for genetic diversity among cattle based on the coat color phenotype.

키워드

저자

• Kyoung-Sub Jung [ Insititute of Livestock and Veterinary Research, Chung Cheong Buk-do 363-931, Korea ]
• Ji-Hye Lee [ Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Korea ]
• Sang-Hwan Kim [ Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Korea ]
• Ho-Jun Lee [ Department of Animal Life Research, Hankyong National University, Anseong 456-749, Korea ]
• Jong-Taek Yoon [ Institute of Genetic Engineering, Hankyong National University, Department of Animal Life Research, Hankyong National University, Anseong 456-749, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636