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Analysis of Bovine Interferon-Tau Genes Expressions in the Promoter Region including ETS2 Binding Site

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.39-39
  • 저자
    Min-Su Kim, Kwan-Sik Min, Ik Soo Jeon, Sung Woo Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347320

원문정보

초록

영어
Regulation of bovine interferon-tau (bIFNT) production, a conceptus secretory protein implicated in the process of maternal recognition of pregnancy, However, its expression in vivo and its transcriptional regulation are not yet well characterized. Among more than bIFNT gene sequences characterized, approximately 90% of bIFNT transcripts expressed in utero is derived from bIFNT1 gene and amounts of transcripts from other bIFNT genes such as new type I bIFNTcl. It was hypothesized that the variation in expression levels exhibited by the bIFNT1 and bIFNTc1 genes was due to identical in the proximal promoter regions of these bIFNT genes. In the present inverstigation, the 5’-upstream (positions ‒1,000 to +51) region of bovine IFNT1 and IFNTc1 genes were analysed using bovine ear-derived fibroblast (EF) cell line, which would provide a more suitable system for studies of the bovine trophoblast transcription factor ETS2. Luciferase assay, the 5’-upstream region (positiond ‒1,000 to +51) of bIFNT1 and bIFNTc1 was then truncated at positions ‒637, ‒389, ‒262, ‒222, or ‒157, or point-mutated at potential ETS-binding site. As compared to the wild-type constructs, those with deletion and mutation constructs were reduced bIFNT transcription similarly. Also, these findings were confirmed by mutated examining idented ETS2 binding site on the bIFNT1 and bIFNTc1 genes 5’-upstream region, ETS2 transcription factor expression constructs revealed the importance of a ETS2 binding site at (between -79 to -70) was idented in the bIFNT1 and bIFNTc1 genes. These results demonstrate these the short promoter region including the ETS2 binding site, gene display identical by transcription factor ETS2, tissue-specific expression and developmental regulation during pregnancy, in determining bovine IFNT genes transcription by the trophectoderm.

키워드

IFNT Transcripts Constructs ETS2 Trophectoderm

저자

  • Min-Su Kim [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]
  • Kwan-Sik Min [ Animal Biotechnology, Hankyong National University, Kyonggi-do, South Korea ]
  • Ik Soo Jeon [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]
  • Sung Woo Kim [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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