Earticle

초록

영어

We determined the optimal polyvinyl Alcohol(PVA) concentration in previous study. However, the sperm progressive motility was lower compared to viability. To improve the motility, we determined the effect of pH adjustment in PVA-extender and post-thaw incubation time on dog sperm viability, motility, reactive oxygen species(ROS) level and gene expression levels related to motility(SMCP) and apoptosis(Bcl-2). Spermatozoa collected from five dogs were resuspended (1×108 cell/mL) with Tris extender 1 containing 0.1, 0.2, 0.3 g PVA with non-adjustment of pH (6.45) and adjustment of pH (6.85), respectively. The extended spermatozoa were cooled to 4℃ for 1 h. The cooled spermatozoa were diluted (1:1) with Tris extender 2 containing 1 M glycerol and PVA concentrations used in the 1st dilution and then loaded in 0.5 mL straws. The straws were kept at 4℃ for additional 30 min and were frozen using LN2 vapor. Sperm viability was assessed after cooling and post-thaw incubation at 24°C for 20 min. Sperm progressive motility was assessed after cooling, post-cooling incubation at 24°C for 20 min, immediately after thawing and post-thaw incubation at 24°C for 20 min. In addition, ROS level and the relative abundance of Bcl-2 and SMCP mRNA(qRT-PCR) were measured following postthaw incubation. As results, adjustment of pH to 6.85 in PVA-Tris extender significantly (p<0.05) improved sperm progressive motility compared to non-adjustment groups with no significant effect on sperm viability, ROS and mRNA expression level of Bcl-2 and SMCP. Post-thaw incubation at 24°C for 20 min significantly improved the progressive motility in all pH adjusted groups. We conclude that adjustment of pH to 6.85 and post-thaw incubation in PVA-Tris extender could considerably improve motility in dog sperm cryopreservation.

키워드

저자

• Sang-Hyoun Park [ Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Iksan 54596, Republic of Korea ]
• Md. Ataur Rahman [ Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Department of Surgery and Radiology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh ]
• AH Nabeel [ Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, Sudan University of Science and Technology, Khartoum, Sudan ]
• Yubyeol Jeon [ Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Iksan 54596, Republic of Korea ]
• Il-Jeoung Yu [ Laboratory of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Iksan 54596, Republic of Korea ]

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간행물

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• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636