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Poster Presentation : Cryopreservation

Cryoprotectant Effect of Trehalose on Frozen-Thawed Boar Sperm

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.31-31
  • 저자
    Baek SY, Choi TJ, Kim YM, Cho ES, Soh HC, Cho KH, Chung HJ
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347314

원문정보

초록

영어
The objective of this study was to investigate the influence of the addition of cryoprotectants to freezing extenders on post thawing boar sperm quality as well as investigate their optimum concentrations. To evaluate the effect of trehalose (0, 80, 100, 120 mM) added in egg yolk extender freezing medium on motion characteristics, motility, viability and DNA integrity of spermatozoa at post thawing of cryopreservation. Semen were collected from 10 boars and frozen on the same day. Qualified semen samples (motility >80%) from each boar were subdivided four groups, 0 (control), 80 mM (T1), 100 mM (T2) and 12 0 mM (T3). Motility was assessed for % motile cell characteristics using computer-assisted semen analysis (CASA; SAIS SI-100, Medical supply, Korea). Frozen boar sperms were thawed in Beltsville Thawing Solution (BTS) then incubated at 38 celsius degree for 20 minutes. Computer progressive motility, average path velocity, straight line velocity, curvilinear velocity and total motility were higher (p<0.05) in adding 120 mM treatment group (T3) as compared with control. Moreover, trehalose addition did not damage DNA integrity of spermatozoa. Nevertheless, trehalose concentration treatment did not affect the viability of the spermatozoa as the concentration increased. The data showed that trehalose as an additive cryoprotectant was able to improve sperm quality without on sperm DNA integrity in cryopreservation.

저자

  • Baek SY [ Swine Science Division, National Institute of Animal Science, RDA ]
  • Choi TJ [ Swine Science Division, National Institute of Animal Science, RDA ]
  • Kim YM [ Swine Science Division, National Institute of Animal Science, RDA ]
  • Cho ES [ Swine Science Division, National Institute of Animal Science, RDA ]
  • Soh HC [ Swine Science Division, National Institute of Animal Science, RDA ]
  • Cho KH [ Swine Science Division, National Institute of Animal Science, RDA ]
  • Chung HJ [ Swine Science Division, National Institute of Animal Science, RDA ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 The 17th International Symposium on Developmental Biotechnology

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