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Poster Presentation : Cryopreservation
The Manufacturing Method of Open Pulled Straw (OPS) using Digital Heating Gun
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- 발행기관
- 한국동물생명공학회(구 한국동물번식학회) 바로가기
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- 간행물
- 발생공학 국제심포지엄 및 학술대회 바로가기
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- 통권
- The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
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- 페이지
- pp.30-30
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A347313
원문정보
초록
- 영어
- Vitrification is a common technique of cryopreservation in animal genetic resources of oocytes or embryos and replacing slow freezing methods. This study examined the factors of preparing open pulled straw (OPU) that could be used for vitrification with digital heating gun (DHG). Thermal performance of heat transfer of DHG is more stable and adjustable than traditional way of heating method like hot-plate or alcohol lamp. Thermal setting value of DHG was adjusted to 250, 350, 400 and 450℃ and production rate of wanted OPS was examined. The thickness of wall and outer diameter of OPU were measured by microscopy. OSP production efficiency of 0.5 mL straw was observed at 0, 66.1, 90.5 and 85.7% for 1~2 sec and 9.5, 33.3, 47.6 and 23.8% for 2~3 sec of heating time. OPS with inner diameter in the range of 200~650 μm was produced with 0.5 mL straw, and OPS with inner diameter in the range of 150~300 μm was obtained with 0.25 mL straw. OPS at the desired length and size could be obtained easily when straw was heated for 1.6~2.3 seconds at 350~400℃. Production rates of 0.25 mL straw were higher than those of 0.5 mL straw, so it was needed more attention to control time and power that were required for optimal heat transfer with 0.5 mL straw. Based on the data obtained in this study, hand-made OPSs could be produced for vitrification devices to freeze COCs, oocytes and embryos of with various size of outer diameter.
저자
간행물 정보
발행기관
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- 발행기관명
- 한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
- 설립연도
- 1976
- 분야
- 농수해양>축산학
- 소개
- 동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.
간행물
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- 간행물명
- 발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
- 간기
- 연간
- 수록기간
- 2004~2018
- 십진분류
- KDC 527 DDC 636
이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 The 17th International Symposium on Developmental Biotechnology
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