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C-Phycocyanin Supplementation during In Vitro Maturation Enhances Pre-Implantation Developmental Competence of Parthenogenetic and Cloned Embryos in Pigs

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.25-25
  • 저자
    Shuang Liang, Jing Guo, Yong Xun Jin, Bao Yuan, Jia-Bao Zhang, Nam-Hyung Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347311

원문정보

초록

영어
C-Phycocyanin (C-PC), a protein from green microalgae, has been suggested to possess various biological activities, including antioxidant and free radical scavenging properties. The aim of the current study was to explore the effects of C-PC on the maturation of porcine oocytes and subsequent developmental competence after parthenogenetic activation and somatic cell nuclear transfer (SCNT) as well as the underlying mechanisms. There was no significant improvement in nuclear maturation rates between the control and C-PC supplementation groups (1, 3, 5, 10 μg/mL). However, supplementation of 5 μg/mL C-PC in the maturation medium significantly increased blastocyst formation and hatching rates after parthenogenetic activation (59.6±3.6% and 33.0±2.6% vs. 49.8±3.5% and 27.4±2.4%, respectively). In addition, the presence of CPC during the maturation period significantly improved blastocyst formation rates and total cell numbers after SCNT (24.8±1.9% and 42.2±3.3 vs. 21.6±2.2% and 39.5±3.4, respectively) compared to the control group. Furthermore, cellular proliferation and the expression of pluripotency-related genes (SOX2 and NANOG) were increased in cloned blastocysts derived from the C-PC supplemented group. Importantly, C-PC supplementation during maturation not only improved cumulus expansion and increased the expression of cumulus expansion-related genes (HAS2, PTX3, and PTGS2), but also enhanced antioxidant capacity, improved mitochondria function, and decreased cathepsin B activity in porcine oocytes. These results demonstrate that C-PC may be useful for improving porcine oocyte quality and subsequent developmental competence in embryos.

키워드

In vitro maturation C-phycocyanin Porcine oocyte Parthenogenetic activation Somatic cell nuclear transfer

저자

  • Shuang Liang [ Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, 130062, China. Department of Animal Science, Chungbuk National University, Cheongju 361-763, Republic of Korea ]
  • Jing Guo [ Department of Animal Science, Chungbuk National University, Cheongju 361-763, Republic of Korea ]
  • Yong Xun Jin [ Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, 130062, China ]
  • Bao Yuan [ Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, 130062, China ]
  • Jia-Bao Zhang [ Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, 130062, China ]
  • Nam-Hyung Kim [ Department of Animal Science, Chungbuk National University, Cheongju 361-763, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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