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Poster Presentation : Cloning

Demecolcine Increases Efficiency of Somatic Cell Nuclear Transfer by Preventing Rupture of Nuclei Donor Spermatogonial Stem Cells in Pigs

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    The 17th International Symposium on Developmental Biotechnology (2017.10)바로가기
  • 페이지
    pp.21-21
  • 저자
    Joohyeong Lee, Donghyuk Hur, Seung Tae Lee, Eunsong Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347307

원문정보

초록

영어
Spermatogonial stem cells (SSC) are promising resources for genetic preservation and restoration of male germ cells in human and animals. However, in our preliminary experiment, it was observed that SSC was fragile when used as nuclei donor for SCNT. This study investigated the potential of porcine SSC as donor nuclei for somatic cell nuclear transfer (SCNT) and developmental competence of SSC-derived cloned embryos. In addition, it was examined whether demecolcine could prevent rupture of SSC during SCNT. SCNT embryos were produced using a standard protocol of our laboratory. After electric activation, SCNT embryos were treated with demecolcine combined with 6-DMAP for 4 h, washed, and then cultured in a porcine zygote medium-3 at 39℃ in a humidified atmosphere of 5% CO2, 5% O2, and 90% N2 for 7 days. When SSC was compared with porcine fetal fibroblast (PFF) in the potential to support embryonic development after SCNT, SSC-derived SCNT embryos showed higher (p< 0.05) developmental competence to the blastocyst stage (53.7%) than PFF-derived embryos (28.6%). Treatment of SSC with demecolcine significantly (p<0.01) inhibited the rupture of SSC during SCNT (7.8% vs. 16.4%) and increased fusion of cell-ooplasm couplets compared to no treatment (71.9% vs. 62.8%). In addition, even after demecolcine treatment, SSC-derived SCNT embryos showed a higher blastocyst formation (46.4%) than PFF-derived embryos (27.8%). Our results demonstrate that porcine SSC are a desirable donor cell type for production of SCNT pig embryos and demecolcine increases production efficiency of cloned embryos by inhibiting rupture of nuclei donor SSC during SCNT.

키워드

Spermatogonial stem cell Demecolcine Somatic cell nuclear transfer Embryonic development Pig

저자

  • Joohyeong Lee [ Institute of Veterinary Science, Kangwon National University, Chuncheon, Korea ]
  • Donghyuk Hur [ College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea ]
  • Seung Tae Lee [ College of Animal Life Science, Kangwon National University, Chuncheon, Korea ]
  • Eunsong Lee [ Institute of Veterinary Science, Kangwon National University, Chuncheon, College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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