Hye Ryoung Heo, Chang Sup Kim, Jeong Hyun Seo, Hyung Joan Cha
언어
한국어(KOR)
URL
https://www.earticle.net/Article/A346318
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원문정보
초록
영어
A functional glycan chip combined with on-chip enzymatic glycosylation was developed to prepare complex glycan sources and to apply glycan -involved applications simultaneously. Here, GM3 trisaccharide, GM2 tetrasaccharide, and GM1 pentasaccharide were successfully direct biosynthesized on lactose-immobilized surface through consecutive three glycosyltransferase reactions. Biosynthesized GM1-related complex glycans were demonstrated to provide the information on substrate specificity of whole cholera toxin. Thus, the proposed on-chip glycan biosynthesis system can provide a new direction toward obtaining complex glycan sources and complex glycan -involved applications such as glycan -protein interaction analysis and glycan biomarker-employed diagnosis.
저자
Hye Ryoung Heo [ School of Interdisciplinary Bioscience and Bioengineering POSTECH, Pohang 37673, Korea ]
Chang Sup Kim [ School of Chemistry and Biochemistry, Yeungnam University, Gyeongsan 38541, Korea ]
Jeong Hyun Seo [ School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Korea ]
Hyung Joan Cha [ School of Interdisciplinary Bioscience and Bioengineering POSTECH, Department of Chemical Engineering, POSTECH, Pohang 37673, Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.