0-GlcNAc modification of X -linked inhibitor of apoptosis regulates its E3 ubiquitin ligase activity towards 0-GlcNAc transferase and cancer cell growth
Hyeon Gyu Seo, Han Byeol Kim, Min Jueng Kang, Ji Young Yoon, Tae Hyun Kweon, Yun Soo Park, Jingu Kang, Jinwoo Jung, Eugene C. Yi, Tae Ho Lee, Jin Won Cho
언어
영어(ENG)
URL
https://www.earticle.net/Article/A346312
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원문정보
초록
영어
X -linked inhibitor of apoptosis (XIAP), an inhibitor of apoptotic cell death, has a RING domain and functions as an E3 ligase, catalysing the ubiquitination of various substrates such as apoptosis-inducing factor, TGF- [3-activated kinase 1, and MEK kinase 2. Here, we identified 0-linked N-acetylglucosamine ( 0-GlcNAc) transferase ( OGT) as a substrate of XIAP. We showed that OGT catalyses the 0-GlcNAc modification of XIAP at serine 406, and this modification regulates the E3 ligase activity of XIAP towards OGT. Substitution of XIAP Ser406 by alanine decreased its E3 ligase activity toward OGT but not toward other substrates. Stable overexpression of XIAP in HCT116 human colorectal carcinoma cells decreased OGT protein levels and inhibited cancer cell growth and invasion. These results suggest that XIAP requires 0-GlcNAcylation in the regulation of its E3 ligase activity toward OGT, which leads to OGT degradation and the inhibition of cancer cell growth.
저자
Hyeon Gyu Seo [ Glycosylation Network Research Center, Yonsei University ]
Han Byeol Kim [ Glycosylation Network Research Center, Yonsei University, Interdisciplinary Program of Integrated OMICS for Biomedical Science, Graduate School, Yonsei university ]
Min Jueng Kang [ Department of Molecular Medlcine and Biopharmaceutjcal Sciences, School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National university ]
Ji Young Yoon [ Interdisciplinary Program of Integrated OMICS for Biomedical Science Graduate School, Yonsei University ]
Tae Hyun Kweon [ Glycosylation Network Research Center, Yonsei University, Interdisciplinary Program of Integrated OMICS for Biomedical Science, Graduate School, Yonsei university ]
Yun Soo Park [ Glycosylation Network Research Center, Yonsei University, Interdisciplinary Program of Integrated OMICS for Biomedical Science, Graduate School, Yonsei university ]
Jingu Kang [ Department of Systems Biology, College of Life Science and Biotechnology, Yonsei Univeristy, Glycosylation Network Research Center, Yonsei University ]
Jinwoo Jung [ Department of Molecular Medlcine and Biopharmaceutjcal Sciences, School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National university ]
Eugene C. Yi [ Department of Molecular Medlcine and Biopharmaceutjcal Sciences, School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National university ]
Tae Ho Lee [ Department of Systems Biology, College of Life Science and Biotechnology, Yonsei Univeristy ]
Jin Won Cho [ Department of Systems Biology, College of Life Science and BIotechnology, Yonsei University, Glycosylation Network Research Center, Yonsei University, Interdisciplinary Program of Integrated OMICS for Biomedical Science, Graduate School, Yonsei university ]
Corresponding author
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
