Earticle

초록

영어

Gastric cancer (GC) is one of the leading causes of cancer-related death worldwide, largely because of difficulties in early diagnosis. In spite of accumulating evidence linking aberrant glycosylation and GC, site-specific localization of the glycosylation for improving clinical specificity and sensitivity is still in analytical challenge. Here, we constructed an analytical platform with targeted glycoproteomic approach for GC biomarker discovery. Unlike conventional glycomic approach characterized by untargeted MS profiling of released glycan, developed platform can be characterized by three key features: target protein-specific, glycosylation site-specific, and structure-specific platform with one-shot non-specific enzyme digestion. Serum haptoglobin (Hp) was enriched by immunoaffinity purification and subjected to pronase to generate site-specific glycopeptides. Glycopeptides were identified and quantified by nano LC/MS and -LC/MS/MS, resulting in full characterization of glycan macro- and micro-heterogeneity. A total of 96 glycopeptides, each corresponding to a unique glycan/glycosite pairing, were tracked across all cancer and control samples. Significant alterations in site-specific glycosylation have been observed in Hp in GC patients.

저자

• Jua Lee [ Asia Glycomics Reference Site, Chungnam National University, Daejeon 34134, Korea Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 34134, Korea ]
• Serenus Hua [ Asia Glycomics Reference Site, Chungnam National University, Daejeon 34134, Korea Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 34134, Korea ]
• Sung Hyeon Lee [ GLYCAN Co., Ltd., Seongnam 13620, Korea ]
• Myung Jin Oh [ Asia Glycomics Reference Site, Chungnam National University, Daejeon 34134, Korea Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 34134, Korea ]
• JaekyungYun [ Asia Glycomics Reference Site, Chungnam National University, Daejeon 34134, Korea Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 34134, Korea ]
• Jin Young Kim [ Department of Food and Nutrition, Chungnam National University, Daejeon 34134, Korea ]
• Jae-Han Kim [ Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea ]
• Jung Hoe Kim [ Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea ]
• Hyun Joo An [ Asia Glycomics Reference Site, Chungnam National University, Daejeon 34134, Korea Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 34134, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614