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Gene cloning and enzymatic characterization of endo -arabinanase type-B from Lactobacillus shenzhenensis

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2018 한국당과학회 동계학술대회 (2018.01)바로가기
  • 페이지
    pp.67-67
  • 저자
    Ji-Eun Park, Yewon Kang, Chang-Yun Choi, Sung Hwan Jeon, Nam Soo Han, Tae-Jip Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A346289

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원문정보

초록

영어
endo-(1,5)-α-L-Arabinanase (ABN, EC 3.2.1.99) is an endo-acting arabinosyl hydrolase that can cleave α-(1,5)-L-arabinofuranosidic linkages in arabinan and arabinooligosaccharides(AOS). In the present study, a putative gene encoding ABN (LbshABN-B) has been found from the genome of Lactobacillus shenzhenensis LY-73 which is isolated from a dairy beverage in China's Shenzhen market. The open reading frame of LbshABN-B consists of total 1,242 bp nucleotides encoding 414 amino acids with a predicted signal peptide of 24 amino acids. The molecular mass of LbshABN-B without signal peptide is 46.2 kDa. It shares approximately 50% of amino acid sequence identities with the other known ABNs type-B, whereas it showed less than 29% of sequence identities with ABNs type-A. The LbshABN-B gene being fused with six-histidines at its C-terminus was constitutively expressed and purified from Escherichia coli. Although the debranched (linear) arabinan is the most preferred substrate, LbshABN-B showed the significant activity towards sugar beet (branched) arabinan as well. This enzyme showed the highest activity on debranched arabinan at 45oC and pH 6.0 in 50 mM sodium acetate buffer, respectively. LbshABN-B can be applicable to the production of various branched AOS from sugar beet arabinan.

저자

  • Ji-Eun Park [ Division of animal, horticultural, and Food Science, Graduate of Chungbuk National University, Cheongju 361-763, Kore ]
  • Yewon Kang [ Division of animal, horticultural, and Food Science, Graduate of Chungbuk National University, Cheongju 361-763, Kore ]
  • Chang-Yun Choi [ Division of animal, horticultural, and Food Science, Graduate of Chungbuk National University, Cheongju 361-763, Kore ]
  • Sung Hwan Jeon [ Division of animal, horticultural, and Food Science, Graduate of Chungbuk National University, Cheongju 361-763, Kore ]
  • Nam Soo Han [ Division of animal, horticultural, and Food Science, Graduate of Chungbuk National University, Cheongju 361-763, Kore ]
  • Tae-Jip Kim [ Division of animal, horticultural, and Food Science, Graduate of Chungbuk National University, Cheongju 361-763, Kore ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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