Jeong Hyun Seo, Chang Sup Kim, Hyung Joon Cha, Hyun Kyoung Lee, JuYeonLee, GunWookPark, JinYoungKim, JongShinYoo
언어
영어(ENG)
URL
https://www.earticle.net/Article/A346286
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원문정보
초록
영어
Mouse has been used as an animal model for scientific research owing to its physiological similarity to human. However, their glycosylation of glycoproteins showed many differences between mouse and human. Usually N-glycosylation is directly involved in various biological process and plays crucial role in human diseases due to their unusual biological sensitivity. Therefore, the site-specific characterization of N-glycosylation in model mouse is necessary in order to investigate progression of disease. This study provided comparison of the site-specific N-and O-glycosylation between human and mouse plasma using LC-MS/MS with GPA (GlycoProteome Analyzer) system1.Thesite-specificN-andO-glycosylationbetweenhumanandmousehasthreedifferencesinpla sma.First,thesialicacidofN-andO-glycopeptideswasalmostentirelyNeu5Gcinmouseplasma,whileinh umanplasmawasNeu5Ac.Second,O-acetylatedNeuGcofN-glycopeptidewasidentifiedinmouseplasm aonly.Third,antennalHexNAc-NeuGcofN-glycopeptidewasspecificallyidentifiedfromseveralglycop roteinsinmouseplasmaonly.Inconclusion,ourstudyhasprovidedanoverviewofthesite-specificN-and O-glycosylationofhumanandmouseinplasma.
저자
Jeong Hyun Seo [ Korea Basic Science Institute, O-chang Cheongju, Korea ]
Chang Sup Kim [ Korea Basic Science Institute, O-chang Cheongju, Korea ]
Hyung Joon Cha [ Korea Basic Science Institute, O-chang Cheongju, Korea ]
Hyun Kyoung Lee [ Korea Basic Science Institute, O-chang Cheongju, Korea, GraduateSchoolofAnalyticalScienceandTechnology,ChungnamNationalUnive rsity,Daejeon,Korea ]
JuYeonLee [ Korea Basic Science Institute, O-chang Cheongju, Korea ]
GunWookPark [ Korea Basic Science Institute, O-chang Cheongju, Korea ]
JinYoungKim [ Korea Basic Science Institute, O-chang Cheongju, Korea ]
JongShinYoo [ Korea Basic Science Institute, O-chang Cheongju, Korea, GraduateSchoolofAnalyticalScienceandTechnology,ChungnamNationalUnive rsity,Daejeon,Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.