Earticle

초록

영어

Violacein is a purple pigment made by enzymatic oxidation and coupling of two molecules of L-tryptophan. It has attracted interest as antileukemic agent and also known for inhibiting the growth of NCI-H460 non-small-cell lung cancer and KM12 colon-cancer cell lines. For most potent antibiotics like avermectin, erythromycin, vancomycin, and doxorubicin, sugars are often essential for the pharmacological properties, especially for water solubility and/or the biological activity of the compounds. Previously increasing solubility of violacein by inclusion complex with β-cyclodextrin and biotransformation by oxidative enzymes to increase the solubility and biological activity of violacein has been reported. The naturally occurring glucosylated violacein analogs have not yet been reported, and no sugar biosynthetic genes have been found in the violacein biosynthetic gene cluster. Violacein glucosides with enhanced solubility and bioactivity is expected, which may be able to fulfill the demand of soluble violacein for in vivo assays. In this study, in order to increase the solubility and enhance the biological activity of violacein we applied two plasmids systems, using the violacein synthesis enzymes (vioABCDE-pETM6), and glycosylation enzyme (Yjic-pET28a). In addition, we tried using the E.coliΔpgiΔzwfΔushA mutant, that has high efficiency in production of UDP-glucose. These studies may be the strategic approach for the production of a glycosylated violacein in reasonable titer.

저자

• Yu Jung Lee [ Department of Life Science and Biochemical Engineering ]
• Jae Kyung Sohng [ Department of Life Science and Biochemical Engineering, Department of BT-Convergent Pharmaceutical Engineering SunMoon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, Korea ]

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간행물

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• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614