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영어

Fucosylation is one of the most common glycosylation and has been associated with the development of hepatocellular carcinoma (HCC). Alpha-fetoprotein (AFP) is clinically used as serological marker to diagnose HCC. Although the level of AFP increased in HCC patients, its sensitivity for diagnosis of HCC is poor because AFP levels are also increased in liver diseases, such as liver cirrhosis. Here, we introduced detection of AFP glycopeptides by parallel reaction monitoring (PRM) mass spectrometry (MS) combined with immunoprecipitation. Also, desialylation of AFP glycopeptides was used to improve MS detection limit (LOD < 2 ng/mL) and to obtain reliable signal (CV < 20%) from 0.1 μL serum. Finally, we compared serum samples from HCC with liver cirrhosis using relative percentage of fucosylated glycopeptide in AFP (AFP-fuc%). AFP-fuc% showed an area under the ROC curve (AUC = 0.949, p value < 0.0001) to discriminate between HCC and liver cirrhosis patients. These results suggest the potential of PRM-based AFP-fuc% in early diagnosis of HCC using serum samples.

저자

• Kwang Hoe Kim [ Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji- Ro, Ochang-eup, Cheongju, 28119, Republic of Korea. Graduate School of Analytical Scienceand Technology,Chungnam National University, Daejeon 34134, Republic of Korea ]
• Heeyoun Hwang [ Graduate School of Analytical Scienceand Technology,Chungnam National University, Daejeon 34134, Republic of Korea ]
• Ju Yeon Lee [ Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, 28119, Republic of Korea ]
• Eun Sun Ji [ Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, 28119, Republic of Korea ]
• Hyun Joo An [ Graduate School of Analytical Scienceand Technology,Chungnam National University, Daejeon 34134, Republic of Korea ]
• Soo-Youn Lee [ Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351,Republic of Korea ]
• Jin Young Kim [ Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, 28119, Republic of Korea ]
• Jong Shin Yoo [ Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji- Ro, Ochang-eup, Cheongju, 28119, Republic of Korea. Graduate School of Analytical Scienceand Technology,Chungnam National University, Daejeon 34134, Republic of Korea ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614