Earticle

초록

영어

Glycan-associated recognition events in cells and organisms are not only critical for diverse physiological processes, but they are also involved in various pathological processes. Therefore, elucidation of glycan-mediated biomolecular interactions and their consequences is of great importance in basic biological research and biomedical applications. In 2002, we and others were the first to employ high-density glycan microarrays in efforts aimed at the high-throughput assessments of glycan-associated recognition events. As they include many glycans attached in a dense and orderly manner to a solid surface, glycan microarrays allow for multiple parallel analyses of glycan-protein binding events by utilizing only small amounts of glycan samples. Therefore, this microarray technology has become a leading edge tool in studies aimed at elucidating roles played by glycans and glycan binding proteins in biological systems. In this presentation, I will discuss our efforts on the construction of glycan microarrays and their applications in studies of glycan-associated interactions. Immobilization strategies of functionalized and unmodified glycans on derivatized glass surfaces will be discussed. Although others have developed immobilization techniques, our efforts have focused on improving the efficiencies and operational simplicity of microarray construction. The microarray-based technology has been most extensively used for rapid analysis of the glycan binding properties of proteins. In addition, glycan microarrays have been employed to determine glycan–protein interactions quantitatively, detect pathogens and rapidly assess substrate specificities of carbohydrate-processing enzymes. More recently, the microarrays have been employed to identify functional glycans that elicit cell surface lectin-mediated cellular responses.

저자

• Injae Shin [ 신인재 | Department of Chemistry, Yonsei University, Seoul 03722, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614