Earticle

초록

영어

Aging is a risk factor for heart disease and heart failure, which result from a progressive impairment of cardiac functions, including stroke volume, cardiac output, blood flow, and oxygen consumption. Age-re-lated cardiac dysfunction is associated with impaired cardiac struc-tures, such as the loss of myocytes, structural remodeling, altered cal-cium (Ca2+) handling, and contractile dysfunction. However, the mecha-nism by which aging affects mitochondrial function in the heart is poorly understood. The purpose of this study was to determine the effects of aging on mitochondrial function in the rat heart. Male Fischer 344 rats were randomly assigned to very young sedentary (VYS, 1 month), young sedentary (YS, 4 months), middle-aged sedentary (MS, 10 months), and old sedentary (OS, 20 months) groups. mitochondrial complex protein levels and mitochondrial function (e.g., mitochondrial hydrogen perox-ide (H2O2) emission and Ca2+ retention capacity) were analyzed in the left ventricle. Aging was associated with decreased levels of OXPHOS (oxidative phosphorylation) protein expression of complex I to IV in the function of the electron transport chain. Aging increased the mitochon-drial H2O2 emitting potential in the heart. In contrast, mitochondrial Ca2+ retention capacity gradually decreased with age. These data demon-strate that aging impairs mitochondrial function in cardiac muscle, sug-gesting that mitochondrial dysfunction with aging may be a primary factor for aging-induced cardiac dysfunction in the heart.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Animals
Preparation of tissue and permeabilized muscle fiber bundles
Mitochondrial H2O2 emission
Mitochondrial Ca2+ retention capacity
Western immunoblot for electron transport chain (ETC) composition in mitochondria
Statistical analyses
RESULTS
Effect of aging on body weight and heart mass
Effect of aging on protein expression of mitochondrial ETC complexes
Effect of aging on mitochondrial H2O2 emission
Effect of aging on mitochondrial Ca2+ retention capacity
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Mi-Hyun No [ Department of Kinesiology, Inha University, Incheon, Korea ]
• Jun-Won Heo [ Department of Kinesiology, Inha University, Incheon, Korea ]
• Su-Zi Yoo [ Department of Kinesiology, Inha University, Incheon, Korea ]
• Han-Sam Jo [ Department of Kinesiology, Inha University, Incheon, Korea ]
• Dong-Ho Park [ Department of Kinesiology, Inha University, Incheon, Korea ]
• Ju-Hee Kang [ Department of Pharmacology and Medicinal Toxicology Research Center, Inha University School of Medicine, Incheon, Korea ]
• Dae-Yun Seo [ Department of Physiology and Cardiovascular and Metabolic Disease Center, Inje University School of Medicine, Busan, Korea ]
• Jin Ha [ Department of Physiology and Cardiovascular and Metabolic Disease Center, Inje University School of Medicine, Busan, Korea ]
• Hyo-Bum Kwak [ Department of Kinesiology, Inha University, Incheon, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613