Earticle

초록

영어

Since the mass mortality of Quercus mongolica has been first reported in Gyeonggi province at 2004, the disease spread rapidly over Korean peninsula annually. Ambrosia beetle (Platypus koryoensis) was known as the insect vector of oak wilt fungus, Raffaelea quercus-mongolicae, and control methods of the disease had mainly been focused on eradication of insect vector. However, for the efficient management of the disease, combined control methods for both of the pathogenic fungus and insect vector are strongly required. As one of the efforts to suppress the pathogenic fungus, antifungal activities of Streptomyces isolated from oak forest soil were assayed in this study. Optimum culture condition for the selected isolates was also studied, As a result, Streptomyces blastmyceticus cultured in PDB (Potato Dextrose Broth) at 25oC for 1 week showed the strongest antifungal activity against oak wilt fungus. Mycelial growth inhibition rates (MGIRs) of Streptomyces isolates were compared on culture media supplemented with heated and unheated culture filtrates of S. blastmyceticus. MGIRs on culture media with unheated culture filtrates were generally higher than those on culture media with heated culture filtrates. Antagonistic mechanism to get involved in the inhibition of hyphal growth and spore formation of the pathogen is due to the antifungal metabolites produced by Streptomyces. This study will provide the fundamental information in developing biocontrol agents for the environment-friendly management of oak wilt disease.

목차

Abstract
Introduction
Materials and Methods
Isolation of endophytic and rhizospheric microorganismsfrom Mongolian oak forest
In vitro screening of the isolated microorganisms against oak wilt fungus
Identification of the selected microorganisms
Optimum culture condition for strong antifungal activityof antifungal isolates against oak wilt fungus
Preparation of culture media containing culture filtrateof antifungal microorganisms and antifungal activity against oak wilt fungus
Lyophilization of antifungal Streptomyces isolates and viability after storage
Results and Discussion
Isolation of endophytic and rhizospheric microorganismsfrom Mongolian oak forest
In vitro screening of the isolated microorganismsa gainst oak wilt fungus
Identification of the selected microorganisms
Optimum culture condition for strong antifungal activityof antifungal isolates against oak wilt fungus
Antifungal activity against oak wilt fungus on culturemedia containing culture filtrate of antifungal isolates
Viablity of antifungal Streptomyces isolates after lyophilization and storage
Acknowledgements
References

키워드

저자

• A Reum Hong [ Tree Pathology and Mycology Laboratory, College of Forest and Environment Science, Kangwon National University, Chuncheon 24341, Republic of Korea ]
• Ji Ho Yun [ Tree Pathology and Mycology Laboratory, College of Forest and Environment Science, Kangwon National University, Chuncheon 24341, Republic of Korea ]
• Su Hee Yi [ Tree Pathology and Mycology Laboratory, College of Forest and Environment Science, Kangwon National University, Chuncheon 24341, Republic of Korea ]
• Jin Heung Lee [ Gyeonggi-Do Forest Environment Research Institute, Gyeonggi Province, Osan 12408, Republic of Korea ]
• Sang Tae Seo [ Department of Forest Diseases and Insects, National Institute of Forest Science, Seoul 02455, Republic of Korea ]
• Jong Kyu Lee [ Tree Pathology and Mycology Laboratory, College of Forest and Environment Science, Kangwon National University, Chuncheon 24341, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634