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Optimization of Conditions for In Vitro Germination of Seeds of Couroupita guianensis Aubl. - A Threatened Tree with Recalcitrant Seeds

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    Journal of Forest and Environmental Science KCI 등재 KCI 등재 바로가기
  • 통권
    제34권 제5호 (2018.10)바로가기
  • 페이지
    pp.388-394
  • 저자
    Manokari M., Mahipal S. Shekhawat
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A343735

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원문정보

초록

영어
An efficient and reproducible protocol for in vitro germination of seeds of Couroupita guianensis has been developed in the present study. Couroupita guianensis is a medicinally important tree and categorized as threatened plant species by the IUCN. The seeds were surface sterilized using 0.1% HgCl2 and inoculated on MS (Murashige and Skoog) and Woody Plants (Llyod and McCown) medium. The seeds with partially removed seed coat responded better (100%) on half strength MS medium as compared to the full (51%), one-fourth strength MS (79%) and the WP medium. Half strength MS medium conjunct with 2.0 mgㆍL-1 indole-3 butyric acid (IBA) was found best among the different types of auxins and cytokinins tested for seed germination, as all the seeds germinated on this medium combination. The seedlings were successfully hardened in the greenhouse and restored in the field with 83% survival rate. The protocol can be used as conservation measure for large scale multiplication of this threatened forest tree species.

목차

Abstract
Introduction
Materials and Methods
Collection and surface sterilization of seeds and experimentaldesign
The culture media
Effects of media strength, light and darkness on in vitro germination of seeds
Hardening and restoration of in vitro seedlings
Results and Discussion
Effects of media and media strength on germinationof seeds of C. guianensis
Effect of growth regulators on seed germination
Influence of photoperiod on germination of seeds
Hardening of seedlings and reintroduction of plantletsin field
Conflict of interest
Conclusion
Conflict of interest
Acknowledgements
Author contribution
References

저자

  • Manokari M. [ Department of Botany, K.M. Centre for Postgraduate Studies, Puducherry 605008, India ]
  • Mahipal S. Shekhawat [ Department of Botany, K.M. Centre for Postgraduate Studies, Puducherry 605008, India ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    Journal of Forest and Environmental Science [산림과학연구]
  • 간기
    계간
  • pISSN
    2288-9744
  • eISSN
    2288-9752
  • 수록기간
    1981~2026
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 526 DDC 634

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