Earticle

초록

영어

For the control of oak wilt caused by Raffaelea quercus-mongolicae, an antifungal microorganism, Streptomyces blastmyceticus, was used as a potential agent. Culture suspension of S. blastmyceticus was injected into Quercus mongolicae in the research forest of Kangwon National University by ChemJetⓇ trunk injection and Macro-infusion at root flare injection. AlamoⓇ (a.i., propiconazole 14.5%), a fungicide currently used for the control of oak wilt in USA, was also treated by both methods to compare the efficacy. For preventive efficacy, culture suspension of the pathogen was inoculated at 1 month after injection of either agent. Tested trees were cut at 3 months after treatment, stained with 1% Fuchsin acid, and then non-conductive area (NCA) and re-isolation frequency (RIF) of oak wilt fungus were compared among treatments. While NCA was the highest as 47.3% in pathogen only treatment, it was the lowest as 16.0% in sterilized water treatment by Macro-infusion. NCAs of Alamo treatment by Macro-infusion and ChemJet injection were 25.3% and 32.1%, respectively. NCA of S.blastmyceticus treatment by ChemJet injection was 32.3%, similar with Alamo treatment’s by ChemJet injection. All treatments by either injection method showed significantly lower NCA compared to the pathogen only treatment. These results indicate that S. blastmyceticus injection shows the preventive efficacy against oak wilt disease by suppressing the growth of pathogen injected. NCA of Macro-infusion injection of sterilized water was lower as 16.0%, compared to 21.3% of ChemJet injection. It means that Macro-infusion is more effective in translocation of sterilized water than ChemJet injection by even distribution. RIF from wood discs of treated trees showed high in pathogen only treatment, but relatively low in S. blastmyceticus treatment. RIF results were correlated with NCA results. From the above results, it was confirmed that S. blastmyceticus showed preventive efficacy against oak wilt disease by ChemJet trunk or Macro-infusion at root flare injection.

목차

Abstract
Introduction
Materials and Methods
Preparation of experiment site and test trees
Preparation of culture suspension of antifungal microorganism
Injection of culture suspension and Alamo
Inoculation of the pathogen
Analysis of non-conductive area(NCA) and re-isolationfrequency(RIF) of the pathogenic fungi
Histological observation of wood tissues of treated trees
Results and Discussion
Comparison of Non-Conductive Area (NCA) among treatments
Re-isolation frequency (RIF) of oak wilt fungus from the wood discs of treated trees
Histological analyses of wood blocks of treated trees
Acknowledgements
References

키워드

저자

• Jin Heung Lee [ Gyeonggi-Do Forest Environment Research Institute, Osan 12408, Republic of Korea ]
• A Reum Hong [ Tree Pathology and Mycology Laboratory, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea ]
• Ji Ho Yun [ Tree Pathology and Mycology Laboratory, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea ]
• Sang Tae Seo [ Department of Forest Diseases and Insects Research, National Institute of Forest Science, Seoul 02455, Republic of Korea ]
• Jong Kyu Lee [ Tree Pathology and Mycology Laboratory, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634