Earticle

초록

영어

An auxin-producing bacterium, Klebsiella michiganensis Jopap-1, was isolated from a button mushroom bed in Buyeo-Gun, Chungcheongnam-Do. The strain Jopap-1 was classified as a novel strain of K. michiganensis based on a chemotaxonomic and phylogenetic analysis. The isolated K. michiganensis Jopap-1 was confirmed to produce indole-3-acetic acid (IAA), which is one of auxin hormones by TLC and HPLC analyses. The maximum concentration of IAA (96.05 mg L-1) was detected in the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 48 h at 35oC by HPLC quantity analysis. A negative relationship between IAA production and pH variation was estimated to show that the increase of IAA caused acidic pH in the culture. The effect of the supplement on L-tryptophan (precursor of IAA) production was observed to be highest at 0.1% concentration, but was significantly lowered above a concentration of 0.2%. To investigate the growth-promoting effects on the crops, the culture broth of E. michiganensis Jopap-1 was infected to water cultures and seed pots of mung bean and lettuce. Consequently, the adventitious root induction and root growth of mung bean and lettuce were observed to be 2.1 and 1.8 times higher than those of the control.

목차

ABSTRACT
서 론
재료 및 방법
균주의 분리
Auxin 생성균주의 선발
TLC에 의한 IAA의 확인
HPLC에 의한 IAA의 정량분석
Bio assay를 통한 식물생장실험
분리 균주의 동정
결과 및 고찰
균주의 분리
분리균의 동정
Auxin 물질의 확인 및 생산능 비교
Bio assay를 통한 식물생장 실험
배양조건별 Auxin 생산능 비교
결 론
REFERENCES

키워드

저자

• 김예슬 [ Ye-Seul Kim | 충남대학교 농업생명과학대학 생물환경화학과 ]
• 윤민호 [ Min-Ho Yoon | 충남대학교 농업생명과학대학 생물환경화학과 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635