This study was conducted to compare the composition and antioxidant activity of 1- and 2-year-old Poria cocos Wolf cultivated at a mortuary and cemetery. An elemental analyzer test showed oxygen, carbon, hydrogen, nitrogen, and sulfur to be present at concentrations of 45~46%, 39~41%, 6.06~6.1%, 0.21~0.22%, and 0%, respectively. No differences in composition were observed among samples. Eleven minerals (S, Ca, Mg, P, As, Se, Cu, Fe, Pb, Zn, and Cd) found in P. cocos cultivated at the mortuary and cemetery were analyzed by inductively coupled plasma mass spectrometry (ICP). The levels of S, Fe, Mg, and Zn in P. cocos were higher in cemetery-cultivated samples than in mortuary-cultivated samples. A 1,1-diphenyl- 2-picrylhydrazyl (DPPH) assay for antioxidant activity revealed half-maximal inhibitory concentration (IC50)values of P. cocos to be 8.601 mg/mL (mortuary, 1 year old), 12.85 mg/mL (cemetery, 1 year old), 1.23 mg/mL (mortuary, 2 years old), and 1.18 mg/mL (landfill, 1 year old). A 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay revealed IC50 values of 15.85 mg/mL (mortuary, 1 year old),14.59 mg/mL(cemetery, 1 year old), 3.9 mg/mL (mortuary, 2 years old), and 14.92 mg/mL (cemetery, 1 year old). The results showed a concentration-dependent effect. Two-year-old mortuary-cultivated P. cocos had the highest antioxidant activity among samples. Ultrastructure analysis with a field emission scanning electron microscope (FESEM) showed no obvious differences among samples.
목차
ABSTRACT 서론 재료 및 방법 복령 시료의 수집 Elemental Analyzer 법에 의한 시설 재배 복령과 토경 재배 복령의 성분 비교 ICP법에 의한 시설과 토경 재배 복령의 성분 비교 복령추출물의 준비 항산화 활성 시험 FRAP assay 환원력 FE-SEM을 통한 시설 재배 복령과 토경 재배 복령의 초미세구조 비교 통계처리 결과 및 고찰 Elemental Analyzer 법에 의한 시설재배 복령과 토경재배 복령의 성분 비교 ICP(inductively coupled plasma mass spectrometry) 항산화활성 FE-SEM을 통한 시설 재배 복령과 토경 재배 복령의 초미세구조 비교 적요 감사의 글 REFERENCES
키워드
Antioxidant activityElementary analysisPhytochemical compoundPoria cocos wolf
저자
김진윤 [ Jin-Yoon Kim | 경북대학교 수의과대학 ]
이화용 [ Hwa-Yong Lee | (주)엔퓨텍 ]
조우식 [ Woo-Sik Jo | 경상북도농업기술원 농업환경연구과 ]
박승춘 [ Seung-Chun Park | 경북대학교 수의과대학 ]
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