Earticle

초록

영어

Peripheral nerve injuries are commonly encountered clinical problem and often result in chronic pain and severe functional deficit. Diosgenin is a plant steroidal saponin and has anti-inflammatory and anticancer effects. In the present study, we investigated the effect of diosgenin on functional recovery following sciatic crushed nerve injury in rats. Walk-ing track analysis for the functional recovery which can be quantified with the sciatic function index (SFI) was conducted. Immunohistochem-istry for c-Fos in the ventrolateral periaqueductal gray (vlPAG) and paraventricular nucleus (PVN) and western blot for brain-derived neu-rotrophic factor (BDNF), tyrosine kinase B (TrkB), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthesis (iNOS) in the sciatic nerve were performed. The right sciatic nerve was crushed for 30 sec using a surgical clip. The animals in the diosgenin-treated groups received oral-ly once a day at the respective doses for 7 consecutive days, starting one day after surgery. Sciatic crushed nerve injury showed character-istic gait changes showing decrease of SFI value. Diosgenin treatment increased the SFI value and suppressed nerve injury-induced c-Fos ex-pression in the vlPAG and PVN. Diosgenin treatment inhibited nerve in-jury-induced increase of BDNF, TrkB, COX-2, and iNOS expressions. It is possible that diosgenin can be used as the new therapeutic agent for pain control and functional recovery following peripheral nerve injury.

목차

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Animals and treatments
  Surgical procedure
  Walking tract analysis
  Tissue preparation
  c-Fos immunohistochemistry
  Western blot analysis
  Data analyses
 RESULTS
  SFI following sciatic crushed nerve injury
  c-Fos expression in the vlPAG following sciatic crushed nerve injury
  c-Fos expression in the PVN following sciatic crushed nerve injury
  BDNF and TrkB expressions in the sciatic nerve following sciatic crushed nerve injury
  COX-2 and iNOS expressions in the sciatic nerve following sciatic crushed nerve injury
 DISCUSSION
 CONFLICT OF INTEREST
 REFERENCES

키워드

저자

• Byung-Ki Lee [ Department of Physical Therapy, Daewon University College, Jecheon, Korea ]
• Chang-Ju Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Mal-Soon Shin [ School of Global Sport Studies, Korea University, Sejong, Korea ]
• Young Sam Cho [ Department of Urology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613