Earticle

초록

영어

To find out the reason why some protein which has specific sequence was not dyed by CBB that is commonly used as dying solution. This can be identify purification of protein like p53Transactivation Domain (TAD) which is not dyed by CBB and help the research of this proteins. After refinement, we're going to figure out through SDS-PAGE why separated TAD protein can't be dyed itself CBB and look for its solution. After finding out a dyeing principle of CBB, we can figure out the reason for not being dyed through analyzing a sequence of TAD. And we find another dying method that in accordance with TAD sequence. We can find that TAD is finally dyed after trying the method of silver staining. The reason why TAD is not dyed by CBB is there is a few amino acids that bind with CBB. We dyed TAD using silver staining that bind with another amino acids in TAD sequence.

목차

ABSTRACT
 1. INTRODUCTION
 2. EXPERIMENTAL DETAILS
 3. RESULTS
 4. DISCUSSION
 5. ACKNOWLEDGEMENTS
 6. REFERENCES

저자

• Taegyun Kwon [ Sejong Science High School, 18-21, Guro-gu, Gung-dong, Seoul, 152-881, Korea ] Corresponding author
• Jaegyung Lee [ Gyungkibuk Science High School, 305 Nok-yang dong, Uijeongbu, 480-120, Korea ]
• Jinhak Kim [ Hansung Science High School, 23-2 Hyunjeo-gil, Seodemoo-gu, Seoul, 120-080, Korea ]
• Sunjin Moon [ Department of Biochemistry, College of Life Science and Biotechnology Yonsei University, Seoul, 120-740, Korea ]
• Weontae Lee [ Department of Biochemistry, College of Life Science and Biotechnology Yonsei University, Seoul, 120-740, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  APEC Youth Scientist Journal [APEC 청소년 과학학술지]

• 간기

  반년간

• pISSN

  2005-5625

• 수록기간

  2009~2022

• 십진분류

  KDC 405 DDC 505